髂内动脉导管化疗对膀胱尿路上皮癌组织中HIF-1α、Ki-67和VEGF表达的影响  

Influence of Iliac Intra-arterial Chemotherapy on Expression of HIF-1α、Ki-67 and VEGF in Urothelial Carcinoma of Bladder

作　　者：张前进[1] 王友宝[1] 韩韬[1] 祝昌明[1] 夏清建[1] 曲江磊[1] 

机构地区：[1]青岛大学医学院附属海慈医院泌尿外科,山东青岛266033

出　　处：《医学信息》2013年第4期82-84,共3页Journal of Medical Information

基　　金：青岛市2010年度医药科研指导计划（2010-WSZD024）.

摘　　要：目的探讨术前髂内动脉导管化疗对膀胱尿路上皮癌组织中HIF-1α、Ki-67和VEGF表达的影响及临床意义，以从分子生物学角度对动脉导管化疗的疗效进行评估。方法采用免疫组化SP法对46例膀胱尿路上皮癌髂内动脉导管化疗前后组织中HIF-1α、Ki-67和VEGF进行检测，并观察化疗后癌组织超微结构的变化。结果动脉导管化疗前后HIF-1α、Ki-67和VEGF的阳性表达率分别为76．1％VS5212％、87．0％VS65．2％和60．9％VS37．0％，均具有统计学差异（P〈O．05）。化疗后癌组织病理分级明显降低，且超微结构观察到的癌细胞有明显受损改变，，结论检测HIF-1α、Ki-67和VEGF对膀胱尿路上皮癌髂内动脉导管化疗的疗效判断有重要意义，髂内动脉导管化疗能明显降低肿瘤标记物的表达，作为一种新辅助化疗方案将为临床推广应用提供理论依据，可成为以后一种可信、有效的综合治疗手段．Objective To investigate the influence of preoperative Iliac Intra-arterial chemotherapy on expression of HIF-1α、Ki-67 and VEGF in urothelial carcinoma of bladder,so as to assess the curative effect of intra-arterial chemotherapy from the level of molecular biology. Methods Expressions of HIF-1α、Ki-67 and VEGF were examined by SP immunohistochemical staining in 46 eases Urothelial Carcinoma of Bladder before and after Intra-arterial chemotherapy.Cancer tissues after Intra-arterial chemotherapy were examined ultramicroscopically. Results Positive rate of HIF-1α、Ki-67 and VEGF expression before and after Intra-arterial chemotherapy were 76.1%VS52.2%,87.0%VS65.2% and 60.9%VS37.0%,They have statistically significantly differences in the positive rate of their expressions between before and after Intra-arterial chemotherapy （P〈0.05）.We found the pathological grading of cancer tissues were reduced obviously after the arterial chemotherapy,and the cancer cells were severe damaged ultramicroscopically .Conclusion Detecting the expression of HIF-1α、Ki-67 and VEGF may help us to esimate the effect of Iliac Intra-arterial Chemotherapy for bladder cancer.And preoperative Intra-arterial chemotherapy can downregulate the expression of the factors mentioned above.It will provide theoretical basis for popularization and application of clinical work as a neoadjuvant chemotherapy method and could be a dependable and efficient comprehensive trealment method later.

关 键 词：动脉化疗 膀胱癌 HIF-1Α KI-67 VEGF 

分 类 号：R737.14[医药卫生—肿瘤]