瑞舒伐他汀钙对慢性梗阻性黄疸大鼠肝脏纤维化的影响  

Treatment with rosuvastatin calcium improves liver fibrosis in a rat model of chronic obstructive jaundice

作　　者：余水平[1] 周雪玲[1] 

机构地区：[1]桂林医学院附属医院肝胆外科,广西壮族自治区桂林市541000

出　　处：《世界华人消化杂志》2012年第35期3564-3569,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨慢性梗阻性黄疸大鼠中瑞舒伐他汀钙对氧化低密度脂蛋白(Ox-LDL)表达的调节及肝脏纤维化影响.方法:90只SD大鼠随机分成对照组(A组)、慢性梗阻性黄疸组(B组)和慢性梗阻性黄疸+瑞舒伐他汀钙组(C组),每组每时段6只.分别于造模后第1、2、3、4和5周抽取大鼠静脉血检查肝功能,免疫荧光法检测Ox-LDL在肝脏的表达,放射免疫法检测肝纤维3项,光学显微镜观察肝组织的病理改变.第4周行胆总管十二指肠吻合再通.结果:B组大鼠术后总胆红素明显升高,组织病理学检查显示肝细胞变性,并有纤维增生性改变.与A组比较,慢性梗阻性黄疸组肝组织Ox-LDL的表达增强,肝纤维3项术后第3周起明显升高.C组大鼠术后胆红素升高,组织病理学检查显示肝脏有纤维增生改变.与B组比较,肝脏Ox-LDL的表达与肝纤维3项减弱.术后Ox-LDL的表达及肝纤维化3项差异有统计学意义(P<0.05).结论:慢性梗阻性黄疸大鼠中瑞舒伐他汀钙能调节Ox-LDL的表达,延缓肝纤维化的发生.AIM：To investigate the effect of treatment with rosuvastatin calcium on expression of Ox-LDL and liver fibrosis in a rat model of chronic obstructive jaundice. METHODS：Ninety Sprague-Dawley rats were randomly and equally divided into three groups：control group, and chronic obstructive jaundice group, and rosuvastatin calcium group. Each group was further divided into five subgroups for testing at different time points. Liver function was determined. Expression of Ox-LDL was tested by immunofluorescence, and liver fibrosis was assessed by radioimmunoassay. Pathological changes in liver tissue were observed by optical microscopy at weeks 1, 2, 3, 4, and 5 after model induction. Choledochoduodenostomy was performed at week 4. RESULTS：Rats with chronic obstructive jaundice showed remarkable jaundice and obvious degeneration and hyperplasia of liver cells. There were also significant changes in expression of Ox-LDL and three liver fibrosis indicators. Jaundice slowly subsided after choledochoduodenostomy. Total bilirubin, expression of Ox-LDL and three liver fibrosis indicators differed significantly at different time points between the chronic obstructive jaundice group and rosuvastatin calcium group （all P 0.05）. CONCLUSION：There was a significant relation between expression of Ox-LDL and liver fibrosis in rats with chronic obstructive jaundice. Treatment with rosuvastatin calcium could regulate the expression of Ox-LDL and improve liver fibrosis in rats with chronic obstructive jaundice.

关 键 词：梗阻性黄疸 氧化低密度脂蛋白 瑞舒伐他汀钙 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统]