Th17与Foxp3^+Treg在胃癌患者外周血中的表达  

Clinical significance of number of Th17 and Foxp3+Treg cells in peripheral blood of patients with gastric cancer

作　　者：叶妮[1] 张光波[2] 高楠[1] 黄语嫣[1] 钱夷婷[1] 李伏超[1] 朱一凡[1] 陈卫昌[1] 

机构地区：[1]苏州大学附属第一医院消化科,江苏省苏州市215006 [2]苏州大学免疫研究所,江苏省苏州市215006

出　　处：《世界华人消化杂志》2012年第36期3689-3694,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81272737;江苏省医学重点实验室课题基金资助项目;No.XK201135~~

摘　　要：目的:研究胃癌患者外周血CD4+CD25+Foxp3+调节性T细胞(Treg)及Th17细胞表达的临床意义.方法:收集胃癌患者(n=77)与健康志愿者(n=20)外周血并分离获取PBMC;流式细胞术分析CD4+CD25+Foxp3+Treg表达;PMA+Ionomycin联合刺激后,流式细胞术检测CD4+IL-17+Th17表达;结合临床资料分析Treg及Th17表达的临床意义.结果:胃癌组患者外周血Th17和Treg表达均显著高于正常组(P<0.05).进一步临床分析发现,胃癌患者外周血中Treg细胞表达水平与胃癌临床分期、肿瘤浸润深度及淋巴结转移有关;未见Th17与胃癌临床分期、浸润深度及淋巴结转移相关.结论:尽管两群Th细胞在胃癌患者体内显著升高,但仅Treg与肿瘤进展相关,Th17的作用尚需进一步探讨.AIM：To analyze the clinical significance of the number of Th17 and CD4^＋CD25^＋Foxp3^＋Treg cells in peripheral blood of patients with gastric carcinoma.METHODS：Peripheral blood samples were collected from patients with gastric cancer（n=77） and healthy volunteers（n=20） and used to separate PBMCs.The number of CD4^＋CD25^＋Foxp3^＋Treg was determined by flow cytometry,and the number of CD4＋IL-17＋Th17 was determined after co-stimulation with PMA and ionomycin.The clinical significance of the number of Treg and Th17 cells in patients with gastric carcinoma was then analyzed.RESULTS：The numbers of Th17 and Treg cells in peripheral blood of patients with gastric cancer were significantly higher than those in normal controls（both P〈0.05）.The number of Treg cells,not Th17 cells,was closely related to degree of tumor infiltration,lymph node metastasis,and tumor clinical stage.CONCLUSION：Although the numbers of both Th17 and Treg cells are significantly higher in patients with gastric cancer,only the latter is related to tumor infiltration.

关 键 词：胃癌 调节性T细胞 TH17 肿瘤分期 

分 类 号：R735.2[医药卫生—肿瘤]