结直肠癌线粒体基因组的微卫星不稳定性  

Mitochondrial DNA microsatellite instability in colorectal cancer

作　　者：崔海宏[1] 黄平[1] 赵婷[1] 张振华[1] 徐伟[1] 王小鹏[1] 韩英[2] 

机构地区：[1]中国人民解放军第305医院消化内科,北京市100017 [2]中国人民解放军北京军区总医院消化科,北京市100700

出　　处：《世界华人消化杂志》2012年第36期3811-3815,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨结直肠癌(colorectal cancer,CRC)线粒体DNA(mitochondrial DNA,mtDNA)非编码区及编码区的微卫星不稳定.方法:选取50例新鲜CRC及相应癌旁组织标本,提取基因组DNA.利用荧光标记聚合酶链反应及短重复序列位点扫描的方法,对线粒体DNA编码区及非编码区微卫星不稳定进行比较分析.结果:共检出19例线粒体微卫星不稳定(mitochondrial microsatellite instability,mtMSI),发生率为38%(19/50),线粒体非编码区微卫星与编码区微卫星不稳定有显著相关性(重要数据列出来P<0.05).结论:结直肠癌患者肠黏膜细胞mtDNA中存在较高频率的微卫星不稳定,在一些结直肠癌的发生中可能起一定作用.AIM：To investigate microsatellite instability in the non-coding region and coding regions of mitochondrial DNA in colorectal cancer.METHODS：Mitochondrial DNA was extracted from 50 cases of fresh CRC samples and matched tumor-adjacent noncancerous tissue.Fluorescence polymerase chain reaction and short tandem repeat locus scanning were conducted to analyze microsatellite instability.RESULTS：Mitochondrial DNA microsatellite instability was detected in 19 of 50（38%） patients with CRC.The microsatellite instability in the non-coding region and coding region of mitochondrial DNA had a significant correlation（χ2=4.929,P=0.026）.CONCLUSION：There is a high frequency of microsatellite instability in mitochondrial DNA of CRC patients,and mitochondrial DNA microsatellite instability may play a role in the occurrence of colorectal cancer.

关 键 词：结直肠癌 线粒体DNA 微卫星不稳定 

分 类 号：R735.3[医药卫生—肿瘤]