急性髓系白血病中RAGE-1基因的表达  

Expression of RAGE-1 Gene in Acute Myeloid Leukemia

作　　者：柴海彦[1] 钱军[1,2] 林江[2] 杨静[2] 李云[2] 王翠竹[2] 陈星星[2] 陈芹[2] 邓兆群[2] 姚冬明[2] 马吉春[2] 

机构地区：[1]山西医科大学第一临床学院,山西太原030001 [2]镇江市第一人民医院血液科,江苏镇江212002

出　　处：《中国实验血液学杂志》2013年第1期20-24,共5页Journal of Experimental Hematology

基　　金：江苏省科技厅临床医学科技专项课题(编号BL2012056);江苏省自然科学基金(编号BK2009206);江苏省"333工程"科研项目资助计划(编号BRA2011085);镇江市社会发展项目(编号SH2010015;SH2011056)

摘　　要：本研究通过检测RAGE-1基因在急性髓系白血病(AML)患者骨髓单个核细胞(BMMNC)中的表达,探讨其表达水平与临床变量的相关性。应用RQ-PCR方法检测94例初诊AML患者BMMNC中RAGE-1基因表达水平,分析其与患者年龄、性别、血象、诊断分型和预后的关系,并比较治疗前后RAGE-1表达量的变化。结果表明:26例(28%)AML患者存在着RAGE-1转录本的过表达(1.34-16.34,中位3.07);RAGE-1过表达阳性组与阴性组在性别、年龄、血象和FAB亚型之间均无显著性差异;不同核型预后分组之间RAGE-1过表达频率无显著性差异,且不同类型核型AML患者中RAGE-1转录本过表达频率也无差异。治疗后获得完全缓解的患者RAGE-1表达水平较治疗前明显降低。RAGE-1过表达组与阴性组患者的总体生存时间并无显著性差异。结论:RAGE-1基因过表达是AML中的一个常见分子事件,但对患者预后并无明显影响。The purpose of this study was to detect the expression of RAGE-1 transcript in the bone marrow mononuclear cells （BMMNC） from patients with acute myeloid leukemia （AML） and to investigate the relationship of RAGE-1 expression level with clinical variables. The expression level of RAGE-1 gene in BMMNC from 94 newly diagnosed AML patients was measured using RQ-PCR. The relationship between RAGE-1 expression level and clinical parameters （age, sex, blood cell counts, diagnosis and prognosis ） was investigated, and the levels of RAGE-1 expression were compared in patients before and after treatment. The results showed that overexpression of RAGE-1 transcript was found in 28% （26/＇94） AML patients （ 1.34 - 16.34, median 3.07）. No significant difference was observed in sex, age, blood parameters and FAB subtypes between the groups with and without RAGE-1 overexpression. There was also no significant difference in the frequency of RAGE-1 overexpression among different cytogenetic risk groups and among the patients with different types of karyotypes. The level of RAGE-1 transcript significantly decreased in those patients obtained complete remission after treatment. The overall survival of AML patients with RAGE-1 overexpression was similar as that in those without RAGE-1 overexpression. It is concluded that RAGE-1 overexpression is a common event in AML, but has no impact on the prognosis of patients.

关 键 词：RAGE-1基因 基因表达 急性髓系白血病 临床变量 

分 类 号：R733.71[医药卫生—肿瘤]