柔红霉素对KG1a细胞增殖及肿瘤相关抗原Eps8表达的影响  

Effects of Daunorubicin on KG1a Cell Proliferation and Eps8 Expression

作　　者：甘金莹[1] 熊文艳[1] 王磊[1] 蔡宋浩[1] 李玉华[1] 

机构地区：[1]南方医科大学珠江医院血液科,广东广州510282

出　　处：《中国实验血液学杂志》2013年第1期49-52,共4页Journal of Experimental Hematology

基　　金：教育部新世纪优秀人才支持计划(编号NCET-09-0087);广东省自然科学基金重点项目(编号9251051501000007);广州市科技计划重点项目(编号12C22121595);教育部留学回国人员科研启动基金(第四批)

摘　　要：本研究旨在观察柔红霉素(DNR)对KG1a细胞株的杀伤抑制作用及新型肿瘤相关抗原表皮生长因子受体通路底物8(Eps8)在KG1a细胞中的表达,在mRNA和蛋白水平探讨DNR作用于KG1a后对Eps8表达的影响。不同浓度DNR作用于KG1a细胞24、48和72 h,用台盼蓝拒染色法检测药物对KG1a细胞的杀伤抑制率,用实时荧光定量PCR法检测Eps8 mRNA转录水平的变化和Western blot法观察Eps8蛋白表达变化。结果表明:随着药物浓度(0.1,0.4μg/ml)的增加及作用时间(24,46,72 h)的延长,DNR对KG1a细胞的杀伤抑制率显著提高(r=0.983,P<0.01);不同DNR药物浓度作用于KG1a 24、48和72 h后,Eps8表达水平以DNR作用浓度和时间依赖方式明显下降(r=0.979,P<0.05)。结论:随药物浓度的增加和作用时间的延长,DNR对KG1a细胞的杀伤抑制率提高,使Eps8表达下降,推测降低Eps8的表达可能是DNR抑制KG1a细胞增殖的机制之一。The aim of this study was to observe the inhibitory effect of daunorubicin on KGla cells and the expression of Eps8 which is a novel tumor-associated antigen with its full name epidermal growth factor receptor pathway substrate 8 （Eps8）, and to explore the effect of daunorubicin on Eps8 expression in KG1 a cells at mRNA and protein levels. The KGla cells were treated with different concentration of daunorubicin for 24,48,72 h, then trypan blue staining was used to detect the inhibitory rate of KGla cells, RQ-PCR and Western blot were used to detect Eps8 mRNA and Eps8 protein expression. The results showed that daunorubicin inhibited the proliferation of KGla cells in a dose and time dependent manner （ r = 0. 983, P 〈 0.01 ）. Daunorubicin could reduce the mRNA and protein levels of Eps8 expression in dose and time dependent manners in KGla cells （r = 0. 979, P 〈 0.05 ）. It is concluded that with the increasing of concentration and time of daunorubicin acting on KGla cells, the cell proliferative inhibitory effect increased and the expression of Eps8 decreased, suggesting that the inhibitory effect of daunorubicin on KGla cell proliferation is realized through downregulation of Eps8 expression.

关 键 词：表皮生长因子受体通路底物8 KG1a细胞 柔红霉素 

分 类 号：R737.71[医药卫生—肿瘤] R730.53[医药卫生—临床医学]