嘌呤霉素氨基核苷大鼠肾病模型与阿霉素大鼠肾病模型的比较  

Comparison of two nephrotic syndrome rat models

作　　者：李小艳[1] 丁洁[1] 王学晶 徐国兵 王素霞 冯泉城[1] 姜丽娜[1] 

机构地区：[1]北京大学第一医院儿科,100034 [2]检验科 [3]电镜室

出　　处：《中华肾脏病杂志》2013年第2期137-141,共5页Chinese Journal of Nephrology

基　　金：国家自然科学基金重点项目（30830105）;国家自然科学基金面上项目（81170657）;北京市自然科学基金（7072080）;高等学校博士学科点专项科研基金（200800010088）

摘　　要：目的比较两种经典的大鼠肾病模型的制模方法及评价指标，为肾病模型的研究提供参考数据。方法30只雄性SD大鼠被随机分为对照组，嘌呤霉素氨基核苷组（PAN组）和阿霉素组（ADR组）。比较3组大鼠的制模方法，评价指标（包括不同时间点大鼠体质量，蛋白尿水平，血清白蛋白浓度，胆固醇浓度，肌酐和尿素氮水平，电镜下足突融合程度等），制模药品不良反应及实验费用等指标，评价两种模型的优缺点。结果（1）成模后PAN组大鼠体质量，毛色等均无明显变化；ADR组表现为脱毛、毛色差、大便稀，且体质量显著低于对照组（P〈0．01）。（2）PAN组制模第10天起24h尿蛋白量显著高于对照组并持续至第15天（P〈0．01）；ADR组制模第15天始24h尿蛋白量显著高于对照组并持续至第21天（P〈0．01）。（3）PAN组制模第10天起血清白蛋白水平显著低于对照组（P〈0．01），制模第15天恢复；血清胆固醇水平在制模第10天显著高于对照组（P〈0．叭），第15天恢复；ADR组血清白蛋白浓度在制模第15天显著低于对照组（P〈0．05），制模第21天恢复；两组大鼠不同时间点血肌酐和尿素氮浓度与对照组差异无统计学意义。（4）PAN组在制模第10天足突宽度显著宽于对照组（P〈0．01）；ADR组在第21天时足突宽度显著宽于对照组（P〈0．05）；（5）PAN组成功诱导1只大鼠（100g）肾病模型费用为ADR组的3．1倍（578．10元比186．94元）。结论两种药物均可成功诱导大鼠肾病模型。PAN有制模方便、不良反应小、组间差异小、急性期发病快、周期短等优点，但价格昂贵；ADR给药途径较困难，不良反应及组间差异大，价格相对便宜。Objective To compared two classical rat models of nephrotic syndrome and to provide some reference data to researchers. Methods Thirty male SD rats were randomly divided into control group, puromycin aminonucleoside-induced nephrotic syndrome （PAN） group and adriamycin- induced nephrotic syndrome （ADR） group. The body weight, twenty four hour proteinuria level, serum albumin concentration, cholesterol concentration, creatinine and urea concentration were measured. The renal pathology change was evaluated. The drug toxic effects, administration methods and the costs were also compared. Results There was no significant difference in body weight and hair color between control group and PAN group. Compared to control group, the body weight of the rats significantly decreased at day 15 and day 21 in ADR group （P 〈 0.01）, accompanied by epilation and diarrhea. Compared to control group, the 24-hour urinary protein levels increased significantly at day10 （P 〈 0.01）, day 15 （P 〈 0.01）, and reached the peak level at day 15 （P 〈 0.01）, day 21 （P 〈 0.01） in PAN group and ADR group respectively. Compared to control group, the serum albumin concentration decreased significantly at day 10 （P 〈 0.01）, and return to normal level at day 15. The serum cholesterol concentration was increased significantly at day 10 （P 〈 0.01） and return to normal at day 15 in PAN group. Compared to control group, the serum albumin concentration was decreased significantly at day 15 （P〈 0.05） and return to normal at day 21 in ADR group. No significant difference of serum creatinine and serum urea nitrogen levels were found among three groups. Compared to control group, the width of foot process increased significantly at dayl0 （P 〈 0.01） and day 15 （P 〈 0.05） in PAN group and ADR group respectively. To successfully induce a nephrotic rat model （per 100 g）, the cost of PAN group was 3.1 times of ADR group （578.10 yuan vs 186.94 yuan）. Conclusions Nephrotic syndrome can be ind

关 键 词：嘌呤霉素 氨基核苷 表柔比星 疾病模型 动物 微小病变肾病 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学]