Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis  

作　　者：Alina Elena PARVU Sandu Florin ALB Alexandra CRACIUN Marian Aurel TAULESCU 

机构地区：[1]Department of Pathophysiology, Faculty of Medicine, University of Medicine and Pharmacy 'luliu Hat ieganu,' Cluj-Napoca 400012,Romania [2]Department of Periodontology, Faculty of Medical Dentistry, University of Medicine and Pharmacy 'luliu Hat ieganum,'Cluj-Napoca 400012, Romani~ [3]Department of Biochemistry, Faculty of Medicine, University of Medicine and Pharmacy 'luliuHatieganu,' Cluj-Napoca 400012, Romania [4]Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural andVeterinary Sciences, Cluj-Napoca 400372, Romania

出　　处：《Acta Pharmacologica Sinica》2013年第2期247-254,共8页中国药理学报（英文版）

摘　　要：Aim： To evaluate the effectiveness of subantimicrobial-dose doxycycline （SDD） as an adjunct to scaling and root planing （SRP） treatment against the nitrosative stress of moderate to advanced chronic periodontitis. Methods： Adults with untreated chronic periodontitis （n=174） were randomly administered SRP＋SDD （n=87） （20 mg of doxycycline twice daily） or SRP＋placebo （n=87） treatment for 3 months. At baseline and after 3 months, the probing depths （PD）, bleeding on probing （BOP） and clinical attachment level （CAL） were measured, and a gingivomucosal biopsy was collected to assay the induction of nitric oxide synthase （iNOS） and 3-nitrotyrosine （3NT）, and blood was collected to assay for total nitrites and nitrates （NOx） and 3NT. Results： Compared to baseline, at the completion of treatment, significant decreases in the levels of tissue iNOS and 3NT and serum NOx and 3NT were observed in both groups. SRP＋SDD yielded a greater reduction in the gingivomucosal and serum nitrosative stress markers than did SRP＋placebo. PD, BOP, and CAL reduction were correlated with the nitrosative stress parameters. Conclusion： On a short-term basis, SDD therapy may be used as an adjunct to SRP treatment against nitrosative stress in moderate to advanced chronic periodontitis.Aim： To evaluate the effectiveness of subantimicrobial-dose doxycycline （SDD） as an adjunct to scaling and root planing （SRP） treatment against the nitrosative stress of moderate to advanced chronic periodontitis. Methods： Adults with untreated chronic periodontitis （n=174） were randomly administered SRP＋SDD （n=87） （20 mg of doxycycline twice daily） or SRP＋placebo （n=87） treatment for 3 months. At baseline and after 3 months, the probing depths （PD）, bleeding on probing （BOP） and clinical attachment level （CAL） were measured, and a gingivomucosal biopsy was collected to assay the induction of nitric oxide synthase （iNOS） and 3-nitrotyrosine （3NT）, and blood was collected to assay for total nitrites and nitrates （NOx） and 3NT. Results： Compared to baseline, at the completion of treatment, significant decreases in the levels of tissue iNOS and 3NT and serum NOx and 3NT were observed in both groups. SRP＋SDD yielded a greater reduction in the gingivomucosal and serum nitrosative stress markers than did SRP＋placebo. PD, BOP, and CAL reduction were correlated with the nitrosative stress parameters. Conclusion： On a short-term basis, SDD therapy may be used as an adjunct to SRP treatment against nitrosative stress in moderate to advanced chronic periodontitis.

关 键 词：nitric oxide nitrosative stress chronic periodontitis scaling and root planing treatment DOXYCYCLINE STOMATOLOGY 

分 类 号：TQ460.72[医药卫生—药物分析学] TD355.4[化学工程—制药化工]