多替泊芬、Photosoft和5-氨基酮戊酸对小鼠S180肿瘤的光动力作用研究  

Effects of Duteroporphyrin,Photosoft and 5-ALA-mediated Photodynamic Therapies on Mouse S180 Sarcoma

作　　者：李文敏[1] 蔡晓军[1] 李言冰[1] 陈文晖 李黎波[1] 罗荣城[1] 

机构地区：[1]南方医科大学南方医院肿瘤科,广州市510515 [2]上海复旦张江光动力药物研发中心

出　　处：《中国激光医学杂志》2013年第1期10-14,64,65,共7页Chinese Journal of Laser Medicine & Surgery

基　　金：广东省科技计划项目(2010B031600246)

摘　　要：目的观察光敏剂多替泊芬、Photosoft和5-氨基酮戊酸(5-Aminolevulinic acid,5-ALA)对小鼠S180肿瘤的光动力治疗疗效,并初步探讨其抗肿瘤机制。方法荷瘤昆明小鼠40只,随机分为4组:A组为多替泊芬-光动力组,多替泊芬药物浓度为10 mg/kg;B组为Photosoft-光动力组20 mg/kg;C组为5-ALA-光动力组,5-ALA药物浓度为100 mg/kg;D组为空白对照组。治疗后观察肿瘤大体形态、体积、细胞凋亡坏死的比例及病理组织学的变化。结果治疗后21 d,各治疗组的肿瘤体积平均值小于空白对照组(P<0.05),治疗组间肿瘤体积平均值Photosoft组小于5-ALA组(P<0.05)。A、B和C组抑瘤率分别为62.48%、75.35%和37.17%。治疗后24 h各治疗组的凋亡及坏死的细胞所占比例明显高于空白对照组(P<0.01)。组织病理学检查显示,各治疗组大范围肿瘤细胞坏死,肿瘤血管破坏及部分坏死灶有中性粒细胞浸润。结论各治疗组均可抑制肿瘤生长,其中Photosoft组比ALA组的抑瘤效果好,与多替泊芬组效果相当。治疗后早期各治疗组杀伤肿瘤细胞效果明显,但部分小鼠未获得治愈,有待作进一步动物实验探究其原因。Objective To observe and evaluate the effects of the photodynamic therapy（PDT） mediated by three different photosensitizers, name- ly, Duteroporphyrin, Photosoft and 5-ALA, on mouse S180 sarcoma and explore their antitumor mechanisms.Methods Kunming mice with subcutaneous S180 sarcoma were randomly divided into four groups： （A） the Duteroporphyrin group （10 mg/kg）, （B） Photosoft group （20 mg/kg）, （C） 5-ALA group （100 mg/kg） and （D） blank control group. The mice in Group A- C were given PDT after tail vein injection of photosensitizers, whereas those in Group D didn~ receive any treatment. After the treat- ment, tumor changes both in appearance and size were observed. The necrosis and apoptosis of tumor cells were quantified with flow cytometry technology. The pathological sections of the tumor tissues were observed via HE staining. Results The tumor size was smaller in the PDT groups than in the control group on the 21st day after the therapy （P 〈 0. 05 ）. As to the three PDT groups, the tumor size was smaller in the Photosoft group than in the 5-ALA group （ P 〈 0. 05 ）. The tumor inhibition rates in the Duteroporphyrin, Photosoft and 5-ALA group were 62. 68%, 75.35% and 37. 17% respectively. Compared with the con- trol group, the proportion of necrotic and apoptotic cells in the PDT groups increased significantly 24 hours after the treatment （ P 〈 0. 01 ）. The pathological changes of tumor tissues in the PDT groups 24 hours after the treatment included ： a wide range of tumor nee- rosis, tumor vascular disruption and neutrophil infiltration. Conclusions Tumor growth inhibition was observed in each PDT group. The effect was stronger in the Photosoft group than in the 5- ALA group while the Photosoft group and Duteroporphyrin group being similar in this respect. The effect of tumor cell necrosis and ap- optosis was remarkable in each PDT group, but some mice didnt get cured. The reason behind is subject to further exploration based on animal experiments.

关 键 词：光动力疗法 多替泊芬 Photosoft 5-氨基酮戊酸 

分 类 号：R730.5[医药卫生—肿瘤]