人牙髓细胞共混物三维生物打印技术  

Three dimensional bioprinting technology of human dental pulp cells mixtures

作　　者：薛世华[1] 吕培军[2] 王勇[2] 赵雨[3] 张婷[3] 

机构地区：[1]北京大学口腔医学院·口腔医院第一门诊部,北京100034 [2]北京大学口腔医学院·口腔医院口腔医学计算机应用中心,卫生部口腔医学计算机应用工程技术研究中心,口腔数字化医疗技术和材料国家工程实验室,北京100081 [3]清华大学机械工程系生物制造工程研究所,先进成形制造教育部重点实验室,北京100084

出　　处：《北京大学学报（医学版）》2013年第1期105-108,共4页Journal of Peking University:Health Sciences

摘　　要：目的:对人牙髓细胞(human dental pulp cells,hDPCs)共混物三维生物打印技术进行初步的探索,为三维生物打印技术应用于牙再生奠定初步基础。方法:用Imageware 11.0软件设计三维生物打印蓝图;制备海藻酸钠-明胶水溶胶,与体外分离、培养的hDPCs共混,共混物中hDPCs的密度为1×106个/mL,海藻酸钠和明胶的终浓度分别为20 g/L和80 g/L;用三维生物打印技术按一定参数进行hDPCs共混物的三维生物打印,观察打印获得的三维结构体,用钙黄绿素-AM和碘化丙啶溶液对结构体进行染色,评价打印后hDPCs的活性,计算细胞存活率;体外培养打印获得的结构体,用Cell Counting Kit-8试剂检测hDPCs在三维结构体中的增殖。结果:利用Imageware 11.0软件设计出了由圆柱状微丝逐层交错堆积构成的具有网格结构的三维结构体数字模型,用三维生物打印技术可构建出具有自定义形状及尺寸的三维结构体,结构体中hDPCs的存活率为87%±2%,经打印处理的hDPCs在三维结构体中仍可增殖。结论:本研究实现了hDPCs共混物的三维生物打印,为三维生物打印技术应用于牙再生奠定了初步基础。Objective：To explore the three dimensional（3D）bioprinting technology, using human den- tal pulp cells （hDPCs） mixture as bioink and to lay initial foundations for the application of the 3 D bio- printing technology in tooth regeneration. Methods： Imageware 11.0 computer software was used to aid the design of the 3D biological printing blueprint. Sodium alginate-gelatin hydrosol was prepared and mixed with in vitro isolated hDPCs. The mixture contained 20 g/L sodium alginate and 80 g/L gelatin with cell density of 1 x ｜06/mL. The bioprinting of hDPCs mixture was carried out according to certain parameters; the 3D constructs obtained by printing were examined; the viability of hDPCs after printing by staining the constructs with calcein-AM and propidium iodide dye and scanning of laser scanning con- focal microscope was evaluated. The in vitro constructs obtained by the bioprinting were cultured, and the proliferation of hDPCs in the constructs detected. Results： By using Imageware 11.0 software, the 3D constructs with the grid structure composed of the accumulation of staggered cylindrical microfilament lay- ers were obtained. According to certain parameters, the hDPCs-sodium alginate-gelatin blends were prin- ted by the 3D bioprinting technology. The self-defined shape and dimension of 3D constructs with the cell survival rate of 87%±2% were constructed. The hDPCs could proliferate in 3D constructs after printing. Conclusion： In this study, the 3D bioprinting of hDPCs mixtures was realized, thus laying initial founda- tions for the application of the 3D bioorintin~ technoloav in tooth regeneration.

关 键 词：生物打印 牙髓 细胞 培养的 组织工程 

分 类 号：R783[医药卫生—口腔医学]