结直肠癌肝转移相关靶基因转录调控网络的构建及基因本体论功能富集分析  

Construction of miRNA-target networks and gene GO analysis of the specific target genes incolorectal cancer with liver metastasis

作　　者：骆成玉[1] 杨鋆[1] 于德明[1] 季晓昕[1] 赵新飞[1] 

机构地区：[1]首都医科大学附属复兴医院普外科,北京100038

出　　处：《中华普通外科杂志》2013年第2期116-119,共4页Chinese Journal of General Surgery

基　　金：北京市教委科技计划面上资助项目（KM200910025022）

摘　　要：目的研究结直肠癌肝脏转移微小RNA（miRNA）表达的差异及相关特性，并对结直肠癌肝脏转移miRNA对应的靶基因和生物信息学特征进行针对性分析。方法收集10例伴有或不伴有肝转移的结直肠癌患者肿瘤组织标本。应用miRNA芯片方法对两组样本的miRNA表达差异情况进行研究，利用软件预测靶基因。进一步构建和分析肝转移结直肠癌相关的miRNA—Target转录调控网络及基因本体论功能模块。结果芯片结果筛选出6种结直肠癌肝转移组较非转移组表达失调的miRNA（上调的miR一224、miR一1236和miR-622；下调的miR一155、miR一342—5p、miR一363）功能团，行使相应功能。结论miR一224作为调控网络。最显著上调的miR-224不但可以与其对应靶基因行使调控作用，而且可以与其他miRNA协同作用于其下游的靶基因的核心，可同时或异时性调控相关的重要基因功能团，进而完成对结直肠癌肝脏转移的转录后水平操控，在肝转移过程中起重要作用。Objective To explore the microRNA expression changes and related characteristics and analyze the corresponding miRNA target genes and their bioinformatics in colorectal cancer with liver metastasis. Methods The fresh specimens of primary CRC were collected in 10 patients during operation, with liver metastasis or without. The miRNA expression levels were compared by miRNA mieroarray between two groups. Then, target genes were identified using target prediction algorithms. The liver metastasis related miRNA-target networks and gene ontology （GO） bioinformatics analysis were further performed. Results A total of six dysregulated miRNAs were identified in colorectal cancer liver metastasis comparing with no metastasis, including 3 up-regulated miRNAs （miR-224, miR-1236, miR-622） and 3 down- regulated miRNAs （miR-155, miR-342-5p, miR-363 ）. miR-224 with most significantly up-regulation played regulation role not only with corresponding target-genes but also downstream genes. Conclusions As a core of the regulation networks, miR-224 can regulate the related gene functional groups simultaneously and asynehronously. It further implements the post-transcriptional regulation and plays a vital

关 键 词：结直肠肿瘤 肿瘤转移 微RNAS 基因调控网络 

分 类 号：R735.3[医药卫生—肿瘤]