不同浓度胡桃醌对HeLa细胞增殖及凋亡的影响  

Effects of different concentrations of juglone on the proliferation and apoptosis of HeLa cells

作　　者：张巍[1] 赵行宇[2] 李妍[1] 徐俊杰[1] 姜艳霞[1] 吕士杰 

机构地区：[1]吉林医药学院生物化学教研室,吉林省吉林市132013 [2]吉林医药学院心理学教研室,吉林省吉林市132013

出　　处：《解放军医学杂志》2013年第2期99-102,共4页Medical Journal of Chinese People's Liberation Army

基　　金：吉林省教育厅"十一五"教育技术研究项目(2009-314);吉林省教育厅"十二五"教育技术研究项目(2011-286)~~

摘　　要：目的观察不同浓度胡桃醌对宫颈癌HeLa细胞增殖及凋亡的影响,探讨胡桃醌的抗肿瘤作用。方法常规体外培养人宫颈癌HeLa细胞24h,加入10、20、50、100、200μmol/L浓度的胡桃醌再培养24h。通过倒置显微镜观察细胞形态学变化,采用MTT法检测细胞增殖情况,流式细胞仪检测细胞周期及细胞凋亡情况,分光光度计检测细胞Caspase-3蛋白活性。结果细胞形态学观察显示,不同浓度胡桃醌均可导致细胞形态学发生改变;MTT实验表明,胡桃醌对HeLa细胞的生长有明显抑制作用,且抑制作用随药物浓度的增加而增强(P<0.05或P<0.01);流式细胞仪检测结果表明,加入胡桃醌培养24h后HeLa细胞出现G2/M期阻滞,10、20、50、100、200μmol/L胡桃醌处理后,HeLa细胞G2/M期的比例分别增加至12.92%、16.23%、23.64%、34.22%、52.64%。Caspase-3活性检测结果显示,不同浓度胡桃醌均可使Caspase-3蛋白活性增加,且具有剂量依赖性。结论胡桃醌可抑制HeLa细胞的增殖,且具有剂量依赖性,有望用于抗肿瘤治疗。Objective To study the effect of different concentrations of juglone on the proliferation and apoptosis of human cervical cancer cells （HeLa）, and explore the anti-tumor effect ofjuglone in vitro. Methods HeLa cells were cultured in vitro for 24 h and then divided into control group and juglone-treated group. The cells in juglone-treated group were cultured again with 10, 20, 50, 100 and 200μmol/L juglone, respectively, for 24h. The morphological changes in HeLa cells were observed with inverted microscope. The proliferation of HeLa cells was assessed detected by methyl thiazolyl tetrazolium （MTT） assay. The cell cycle and apoptosis were observed by flow cytometry （FCM）. The expression of caspase-3 in HeLa cells was determined by caspase-3 colorimetric assay kit. Results Under morphological observation, it was found that different concentrations of juglone led to change in cell shape. MTT assay showed that juglone significantly inhibited the growth of HeLa cells in a dose-dependent manner （P〈0.05 or P〈0.01）. FCM assay indicated that the percentage of the cell cycles arresting at G2/M phase after treatment with above concentrations ofjuglone for 24 h were increased to 12.92%, 16.23%, 23.64%j 34.22% and 52.64% from 7.5%, respectively （P〈0.05 or P〈0.01）. The caspase-3 activity in juglone-treated HeLa cells remarkably increased in a dose-dependent manner as compared with control group. Conclusion Various concentrations of juglone can inhibit the proliferation and induce the apoptosis of HeLa cells in a dose-dependent manner, implying that juglone may have anti-tumor effect.

关 键 词：胡桃醌 HELA细胞 细胞凋亡 细胞周期 

分 类 号：R737.33[医药卫生—肿瘤]