重组质粒pEGFP-N1-EMMPRIN对胃癌SGC-7901细胞侵袭和迁移的影响  

Effect of recombinant plasmid pEGFP-N1-EMMPRIN on invasion and migration of gastric cancer SGC-7901 cells

作　　者：亢渝俊[1] 王川[1] 姜政[1] 王丕龙[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《上海交通大学学报（医学版）》2013年第2期155-161,共7页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的构建人细胞外金属蛋白酶诱导因子(EMMPRIN)重组质粒,观察其在胃癌SGC-7901细胞中的表达及对细胞侵袭和迁移的影响。方法从结肠癌细胞株SW-480中提取总RNA,通过RT-PCR获得EMMPRIN基因,连接至载体pEGFP-N1,构建重组质粒pEGFP-N1-EMMPRIN,通过脂质体Lipofectamine 2000转染胃癌SGC-7901细胞;RT-PCR和Western blotting分别检测EMMPRIN mRNA和蛋白在SGC-7901细胞中的表达,Transwell实验检测EMMPRIN对SGC-7901细胞侵袭和迁移的影响。结果重组质粒pEGFP-N1-EMMPRIN构建成功,并在SGC-7901细胞中表达,RT-PCR和Western blotting检测示转染后SGC-7901细胞中EMMPRIN mRNA和蛋白表达均增加,Transwell实验检测示转染后SGC-7901细胞的侵袭和迁移能力均显著增强。结论EMMPRIN可显著增强肿瘤细胞的侵袭与迁移能力。Objective To construct the recombinant plasmid encoding human extracellular matrix metalloproteinase inducer （EMMPRIN）, and investigate its expression and effect on invasion and migration of gastric cancer SGC-7901 cells. Methods Total mRNA was extracted from human colon cancer SW-480 cells, EMMPRIN gene was obtained by RT-PCR and cloned into pEGFP-N1 vector, then the recombinant plasmid pEGFP-N1-EMMPRIN was constructed and transfected into human gastric cancer SGC-7901 cells by Lipofectamine 2000. The expression of EMMPRIN mRNA and protein in SGC-7901 cells was detected by RT-PCR and Western blotting respectively, and the effect of EMMPRIN on invasion and migration of SGC-7901 cells was determined with Transewell assay. Results The recombinant plasmid pEGFP-N1-EMMPRIN was constructed and expressed in SGC-7901 cells. RT-PCR and Western blotting indicated that the expression of EMMPRIN mRNA and protein in SGC-7901 cells increased after transfection, and Transwell assay revealed that the invasion and migration of SGC-7901 cells were significantly enhanced after transfection. Conclusion EMMPRIN can significantly enhance the invasion and migration of tumor cells.

关 键 词：重组质粒 细胞外金属蛋白酶诱导因子 SGC-7901 侵袭 迁移 

分 类 号：R735.2[医药卫生—肿瘤]