葛根总黄酮对骨髓瘤细胞株U266及RPMI8226增殖作用的研究  

Study on effects of puerariae radix flavones on the proliferation-of multiple myeloma cell lines U266 and RPMI 8226

作　　者：徐晓度[1] 沈群[1] 季建敏[1] 季鸥[1] 杨月艳[1] 朱光荣[1] 吴萸[1] 陈婷[1] 李彦丽[1] 

机构地区：[1]南京中医药大学第一附属医院血液科, 210029

出　　处：《白血病．淋巴瘤》2013年第1期42-46,共5页Journal of Leukemia & Lymphoma

基　　金：基金项目：国家自然科学基金（81274139）;高等学校博士学科点专项科研基金（20103237110005）

摘　　要：目的初步探讨葛根总黄酮（PRF）对骨髓瘤细胞株U266及RPMI8226增殖影响及其机制。方法采用0、10、30、50、100Ixg／mlPRF分别处理U266、RPMI8226细胞48h及72h，MTF法检测细胞增殖抑制率，流式细胞术检测细胞周期变化，瑞特染色观察细胞形态学改变，FITC—AnnexinV／PI双染法检测细胞早期凋亡率改变，DNA片段化分析观察PRF处理U266细胞DNA断裂片段。结果PRF可以抑制2种骨髓瘤细胞增殖，对U266细胞抑制作用大于对RPMI8226细胞的作用，呈浓度依赖性；随PRF浓度增加2种细胞凋亡比例增加。瑞特染色未观察到2种细胞凋亡形态学特征。0、10、30、50、100Ixg／mlPRF处理U266细胞48h早期凋亡率分别为（3．20±0．36）％、（5．20±0．92）％、（7．30±1．22）％、（8．10±0．53）％、（10．80±0．90）％，呈剂量依赖性增高，组间差异有统计学意义（P〈0．05）。DNA片段化分析U266细胞未出现凋亡细胞特有的DNA梯带。结论一定浓度PRF对骨髓瘤U266及RPMI8226细胞具有较明显的增殖抑制作用；PRF对U266细胞增殖抑制作用机制虽可能与凋亡相关，但并非U266细胞增殖受抑的主要途径，其确切机制有待进一步探讨。Objective To investigate the effects on proliferation of multiple myeloma cell lines U266 and RPMI 8226 induced by puerariae radix flavones （PRF） in vitro and its possible mechanism. Methods Exposed to 0, 10, 30, 50, 100 μg/ml PRF for 48 h and 72 h, the U266 and RPM! 8226 cells proliferation inhibitory rates were detected by M3T assay, cell cycles by flow cytometry （FCM）, morphologic changes of U266 cells by Wright＇ s staining, and early-stage apoptotic rates of U266 cells by FITC-Annexin V/PI staining with FCM. Analysis of DNA fragment was made to test characteristic apoptosis DNA ladder in U266 cells. Results 0, 10, 30, 50, 100 μg/ml PRF could inhibit the proliferation of U266 and RPMI 8226 ceils in a dose-dependent manner （U266 〉 RPMI 8226）. Cell cycle analyses in U266 and RPMI 8226 cells showed that sub-diploid peaks, but cell cycles changed minor. Wright＇s staining of U266 cells showed hardly any apoptostic character istic. Annexin V/PI double staining indicated that early-stage apoptotic rates of U266 cells exposed to O, 10, 30, 50, 100 μg/ml PRF for 48 h were mildly increased in a dose-dependent manner. They were （3.20±0.36） %, （5.20±0.92） %, （7.30±1.22） %, （8.10±0.53） % and （10.80±0.90） %, respectively. The group differences had statistical significance （P 〈 0.05）. Analysis of DNA fragment barely exhibited the characteristic DNA ladder in U266 cells. Conclusion A certain concentrations of PRF could inhibit the proliferation of U266 and RPMI 8226 cells significantly. It is suggested that apoptosis related to the proliferative inhibition mechanism induced by PRF in U266 cell line, but not main. Other pathways such as necrosis and autophagy whether or not involved need further investigation.

关 键 词：多发性骨髓瘤 葛根总黄酮 U266细胞 RPMI8226细胞 

分 类 号：R733.3[医药卫生—肿瘤]