酶解淫羊藿总黄酮制备宝藿苷Ⅰ  

Preparation of baohuosideⅠ by enzymolysis of Epimedium flavonoids with β-glucosidase

作　　者：蒋艳荣[1,2] 张振海[1] 孙娥[1] 陈玲玲[1,2] 崔莉[1,2] 贾晓斌[1,2] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室,江苏南京210028 [2]南京中医药大学,江苏南京210046

出　　处：《中国医院药学杂志》2013年第4期260-263,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(No.30572372;No.30973944);江苏省医药高技术计划项目(编号:BG2007614)

摘　　要：目的:研究酶解淫羊藿总黄酮制备宝藿苷Ⅰ的工艺。方法:采用β-葡萄糖苷酶酶解淫羊藿总黄酮制备宝藿苷Ⅰ,以宝藿苷Ⅰ得率为指标,通过单因素考察pH值、温度、反应时间及酶与底物质量比对宝藿苷Ⅰ得率的影响。结果:酶解反应的最适条件为温度50℃、pH5.0～5.5、反应时间7 h、酶与底物质量比为1∶20;以淫羊藿总黄酮计,宝藿苷Ⅰ收率为9.25%。结论:β-葡萄糖苷酶酶解淫羊藿总黄酮制备宝藿苷Ⅰ,得率高、工艺简便可靠、反应条件温和,适合工业化生产。OBJECTIVE To study the hydrolysis technology of Epimedium flavonoid by β-glucosidase for preparing baohuo side I. METHODS Taking the yield of baohuoside I as the index, β-glucosidase was used for enzymolysis of Epimedium fla vonoids and the effects of pH value, temperature,reaction time and the ratio of enzyme to substrate on yield of baohuoside I were studied. RESULTS The optimum enzymolysis with β-glucosidase was achieved at 50 ℃, a pH 5.0 - 5.5 Solution for7 h, and the ratio of enzyme to substrate is 1：20. The yield of baohuoside I was 9. 25% （Epimedium flavonoid was computed）. CONCLUSION It is feasible to prepare baohuoside I by enzymolysis with β-glucosidase, the condition is moderate and it is suitable for industrialization.

关 键 词：宝藿苷Ⅰ 淫羊藿总黄酮 Β-葡萄糖苷酶 

分 类 号：R95[医药卫生—药学]