错配修复基因hMLH1、hMSH2蛋白在宫颈疾病中的表达  

Expressions of Mismatch Repair Genes hMLH1 and hMSH2 Proteins in Cervical Diseases

作　　者：陶萍萍[1,2,3] 王运根[1,2,3] 郑伟平[1,2,3] 单江静[1,2,3] 黄雅琴[1,2,3] 

机构地区：[1]绍兴市人民医院 [2]绍兴文理学院附属第一医院 [3]浙江大学绍兴医院,浙江绍兴321000

出　　处：《肿瘤学杂志》2013年第2期125-128,共4页Journal of Chinese Oncology

基　　金：绍兴市科技局社会发展科研项目(2007A33007)

摘　　要：[目的]分析不同级别宫颈癌前病变及宫颈癌中错配修复基因hMLH1和hMSH2蛋白表达情况。[方法]收集宫颈活检及手术标本139例,其中宫颈上皮内瘤变(CIN)Ⅰ级43例,CINⅡ、Ⅲ级48例,宫颈鳞癌(SCC)48例;对照组慢性宫颈炎50例。用免疫组化技术检测hMLH1和hMSH2蛋白,分析其与宫颈病变级别及宫颈癌临床病理特征关系。[结果]hMLH1阳性率在CIN各组明显高于慢性宫颈炎及SCC组,差异有统计学意义(P<0.01)。hMSH2阳性率随宫颈病变级别增加而升高,差异有统计学意义(P<0.01);但SCC组与CIN各组相比差异无统计学意义。hMLH1阳性率随FIGO分期增加而下降,但差异无统计学意义;hMSH2阳性率随FIGO分期增加而上升,且与淋巴转移及病理分级有关(P<0.05)。两者与肿瘤大小均无关。[结论]错配修复基因hMLH1、hMSH2蛋白与宫颈癌发生发展有关,但两者作用机制可能不同。[Purpose] To investigate expressions of mismatch repair genes hMLH1 and hMSH2 proteins in cervical intraepithelial neoplasia（CIN） and cervical cancer. [Methods A total of 139 cases of cervical sample tissues from operation or biopsy were collected, including 43 cases of cervical intraepithelial neoplasia （CIN） 1,48 cases of CIN lI and HI ,and 48 cases of squamous cell carcinoma （SCC）. Fifty cases of chronic cervicitis were adopted as control group. Expres- sions of hMLH1 and hMSH2 were detected immunohistoehemieally. The relationship of hMLH1 and hMSH2 with different CIN and clinicopathological features of cervical cancer were ana- lyzed. [Resultsl The positive rates of hMLH1 in CINs were significantly higher than those in chronic eervieitis and SCC,with significant difference （P〈0.01）. The positive rate of hMSH2 in- creased with cervical lesion progress （P〈0.01）,but no significant difference was found between CINs and SCC. The positive rate of hMLH1 declined with the increasing of FIGO stages without significant difference.The positive rate of hMSH2 increased significantly with the increasing of FIGO stages,and were associated with lymph nodes metastasis and pathologic grades （P〈0.05）. Both of the two proteins had no relation with the size of tumor. [Conclusion] Mismatch repair genes hMLH1 and hMSH2 proteins seem to relate with carcinogenesis and progress of cervical cancer,but may have different mechanisms.

关 键 词：错配修复基因 宫颈肿瘤 宫颈上皮内瘤样病变 

分 类 号：R737.33[医药卫生—肿瘤]