Single and Combined Effects of Estrone and 17 β-Estradiol on Male Goldfish  

作　　者：YAN Zhen Hua LU Guang Hua YANG Xiao Fan 

机构地区：[1]Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education,College of Environment,Hohai University

出　　处：《Biomedical and Environmental Sciences》2013年第3期176-184,共9页生物医学与环境科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (51079049);Qing Lan Project of Jiangsu Province

摘　　要：Objective To assess the single and combined effects of estrone （El） and 1713-estradiol （E2） on goldfish （ Carassius auratus）. Methods Batch tests were conducted. Serum levels of vitellogenin （VTG） and E2, gonadosomatic indices （GSI）, gonadal DNA damage and liver 7-ethoxyresorufin-O-deethylase （EROD） activity were measured after exposure for 14 days. Results The VTG level increased significantly in a concentration-dependent manner. The serum E2 level was significantly higher and the GSI level was significantly lower in goldfish after exposed to the 3 drugs. DNA damage occurred in treated samples and EROD activity was significantly suppressed 7 days after exposure. The joint effect of E1 and E2 was additive with regard to VTG induction. Conclusion The results of our study highlight a series of effects of steroidal estrogens on goldfish. Further study is needed to confirm their effect as a whole.Objective To assess the single and combined effects of estrone （El） and 1713-estradiol （E2） on goldfish （ Carassius auratus）. Methods Batch tests were conducted. Serum levels of vitellogenin （VTG） and E2, gonadosomatic indices （GSI）, gonadal DNA damage and liver 7-ethoxyresorufin-O-deethylase （EROD） activity were measured after exposure for 14 days. Results The VTG level increased significantly in a concentration-dependent manner. The serum E2 level was significantly higher and the GSI level was significantly lower in goldfish after exposed to the 3 drugs. DNA damage occurred in treated samples and EROD activity was significantly suppressed 7 days after exposure. The joint effect of E1 and E2 was additive with regard to VTG induction. Conclusion The results of our study highlight a series of effects of steroidal estrogens on goldfish. Further study is needed to confirm their effect as a whole.

关 键 词：VITELLOGENIN DNA damage EROD activity Carassius auratus 

分 类 号：X174[环境科学与工程—环境科学]