尿脱落细胞液基薄层细胞学检测联合DNA定量分析法筛查泌尿系统肿瘤  

Urinary cancer screening by urine thin-layer cytology test and DNA image cytometry

作　　者：马述春 姜玮 王彤瑶 杨柏帅[2] 戴波[2] 王达 

机构地区：[1]上海御康医院,上海201204 [2]上海肿瘤医院,上海200032

出　　处：《中国肿瘤临床与康复》2013年第2期131-133,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：上海市浦东新区卫生科技发展专项基金项目(PW2009A-32)

摘　　要：目的探讨尿脱落细胞薄层细胞学检测(TCT)联合细胞定量DNA分析技术筛查泌尿系统恶性肿瘤的作用。方法在284例正常健康体检人群、102例普通泌尿系统门诊患者及29例膀胱癌患者中,取尿液的脱落细胞,以TCT技术制片,同时行细胞病理检查和DNA定量分析,计算各组的阳性率,各组间的检出率以2比较统计学意义。结果体检组肿瘤细胞0例,可疑细胞1例,DNA定量分析阳性1例,可疑2例;门诊组未见肿瘤细胞,DNA定量分析可疑3例;膀胱癌组肿瘤细胞阳性率为79.3%(23/29),DNA定量分析阳性率为82.8%(24/29)。膀胱癌组尿肿瘤细胞和DNA定量分析阳性率高于体检组和门诊组,2分析表明差异有统计学意义(P<0.01)。结论在没有血尿等临床表现和影像学异常发现前,TCT联合DNA定量分析筛查泌尿系统恶性肿瘤效果明确,简便易行。Objective To evaluate cancer detection by urine thin-layer cytology test （TCT） and DNA-ICM（Image cytometry）. Methods Urine exfoliated cells obtained from 284 healthy cases, 102 out- patients, and 29 bladder cancer patients, processed by TCT technique, then cytopathology diagnosis and au- tomated DNA-ICM were engaged respectively. Positive rates among the groups compared by chi-square test. Results No cancer cell was found in healthy cases and outpatients. None in healthy cases and 1 in outpa- tients DNA-ICM was positive. In 29 bladder patients, 23 cases （79. 3 % ） were found cancer cells, 24 cases （82. 8% ）DNA-ICM were positive. Both cytopathology diagnosis and DNA-ICM positive rates were higher than healthy cases as well as than outpatients significantly（P 〈 0. 01 ）. Conclusions TCT combining DNA- ICM could be an easy measure to screen urinary cancer in persons without clinical findings.

关 键 词：尿液细胞学 薄层细胞学检测 DNA定量分析 膀胱肿瘤 

分 类 号：R737.1[医药卫生—肿瘤]