血管内皮生长因子在脑膜瘤及瘤周脑组织中的表达  

Expression of vascular endothelial growth factor in human meulngiomas and peritumoral brain areas

作　　者：丁鸭锁[1] 钱硕[1] 季卫阳[1] 王汉东[2] 鲁晓杰[1] 

机构地区：[1]南京医科大学附属无锡第二医院神经外科,214002 [2]南京军区南京总医院神经外科

出　　处：《肿瘤研究与临床》2013年第2期100-103,共4页Cancer Research and Clinic

摘　　要：目的研究血管内皮生长因子（VEGF）在脑膜瘤瘤周水肿形成中的作用。方法对经手术证实的幕上脑膜瘤37例患者共40个脑膜瘤进行研究，通过免疫组织化学法及Westernblot检测肿瘤组织和瘤周脑组织中VEGF的蛋白表达，通过反转录PCR法检测VEGF的mRNA表达情况，以与β-actin的比值为其相对表达量；通过水肿指数（EI）评估术前瘤周水肿的严重程度，并分析其与VEGF表达的相关性。结果在VEGF阳性病例中，VEGF蛋白水平在肿瘤组织、瘤周水肿脑组织和正常脑组织之间呈梯度降低（相对表达量分别为0．38±0．08、0．20±0．03、0．04±0．02）。在肿瘤组织中，VEGF的蛋白水平、RNA水平和EI呈正相关（r=0．892、0．875，均P〈0．05）；在瘤周脑组织中，仅VEGF的蛋白水平和EI呈正相关（r=0．912，P〈0．05），而RNA则几乎未表达（0．06±0．02）。结论VEGF在脑膜瘤瘤周水肿的形成过程中可能起着重要作用；VEGF可能是由肿瘤组织产生的，并扩散到瘤周脑组织中形成水肿。Objective To explore the formation mechanism of peritumoral brain edema （PTBE） by vascular endothelial growth factor （VEGF）. Methods 40 biopsies were obtained from 37 patients. Immunohistochemieal staining and Western blot were performed to detect the expression of VEGF protein. Reverse-transcriptase polymerase chain reaction （RT-PCR） was used to analyze the presence and quantity of VEGF mRNA. The extent of PTBE was estimated as an edema index （EI） based on preoperative magnetic resonance imaging. Results In VEGF-positive cases, a decreasing gradient of VEGF protein expression was observed with increasing distance from tumors （0.38±0.08, 0.20±0.03, 0.04±0.02）. In meningiomas, the protein level and the mRNA level were congruent and the expression of both protein and mRNA had a significant correlation with EI （protein： r = 0.892, RNA： r = 0.875, P 〈 0.05）. However, in peritumoral areas, protein level were not consistent with the mRNA level. Protein results showed high correlation with EI （r= 0.912, P 〈 0.05）, but mRNA almost was almost undetectable （0.06±0.02）. Conclusion VEGF is impartant on PTBE. It is concluded that VEGF macromolecules are secreted by tumor tissue and enter peritumoral normal brain tissue to induce edemagenesis in meningiomas.

关 键 词：脑膜瘤 瘤周水肿 血管内皮生长因子类 

分 类 号：R739.45[医药卫生—肿瘤]