青蒿素类药物对结肠腺癌细胞LS174T的细胞毒作用  

Cytotoxicity of artemisinin drugs on colon cancer cells LS174T

作　　者：王燕[1] 薛丹[1] 王锐利[1] 张淑秋[1] 

机构地区：[1]山西医科大学药学院临床药学教研室,山西太原030001

出　　处：《中国医药导报》2013年第7期14-16,共3页China Medical Herald

基　　金：国家自然科学基金资助项目(项目编号:30973589);山西省留学人员管理委员会办公室科研资助项目(项目编号:2009-47)

摘　　要：目的考察青蒿素(ART)、双氢青蒿素(DHA)及蒿甲醚(AM)对结肠腺癌细胞LS174T增殖的影响,为建立青蒿素类药物自身诱导代谢机制的体外研究体系奠定基础。方法采用噻唑蓝(MTT)法检测不同浓度药物、分别作用不同时间后对细胞增殖的抑制情况,计算抑制率并求得半数抑制浓度(IC50)。结果抑制率均随药物浓度的增大和时间的延长而增加,ART作用24、48、72 h对LS174T的IC50分别为>240.00μmol/L、>240.00μmol/L、211.18μmol/L,DHA对LS174T的IC50分别为91.46、80.45、45.60μmol/L,AM对LS174T的IC50分别为>320.00、262.90、163.93μmol/L。结论 3种药物对LS174T增殖的抑制程度不同,但均呈浓度依赖性和时间依赖性。Objective To explore the cytotoxicity effect of artemisinin （ART）, dihydroartemisinin （DHA）, and artemether （AM） on colon cancer cells LS174T, which could provide guidance for establish the in vitro experimental assessment system of autoinduction metabolism mechanism. Methods M3T assay was used to evaluate the inhibition to proliferation of LS174T cells treated by artimisinin drugs （ARTs） of different concentrations and for different time. The inhibition rates and IC50 values were calculated based on the results. Results The inhibition rates increased as dose of ARTs and exposure time increased. When treated for 24, 48 and 72 h, the IC50 values of ART were 〉 240.00, 〉 240 and 211.18 μmol/L, respectively, ICso values of DHA were 91.46, 80.45 and 45.60 μmol/L, respectively, and IC50 values of artemether were 〉 320.00, 262.90 and 163.93 μmol/L, respectively. Conclusion The extents of LSI74T proliferation inhibition caused by ART, DHA and AM are different, but they all exhibit dose-dependent and time-dependent characteristics.

关 键 词：噻唑蓝 青蒿素 双氢青蒿素 蒿甲醚 LS174T 

分 类 号：R285.5[医药卫生—中药学]