HPLC法检测口服液类保健食品中苯甲酸和山梨酸的含量  

Determination of Benzoic Acid and Sorbic Acid in Health Food by HPLC

作　　者：李慧敏 

机构地区：[1]湖南省岳阳市食品药品检验所,岳阳414000

出　　处：《中国药事》2013年第1期38-40,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立HPLC法检测口服液类保健食品中苯甲酸和山梨酸的含量。方法采用Agilent ZOBAXC18柱(4.6mm×250mm,5μm),柱温为30℃;流动相为甲醇-25mmol.L-1乙酸铵(5∶95);流速为1.0mL.min-1;检测波长为230nm。分别以外标法计算含量。结果苯甲酸浓度在1.209～60.43μg.mL-1范围内呈良好线性关系,r=0.9999;平均回收率为103.8%,RSD为1.7%。山梨酸浓度在1.010～50.51μg.mL-1范围内呈良好线性关系,r=0.9998;平均回收率为95.0%,RSD为1.9%。结论本方法可用于测定口服液类保健食品中苯甲酸、山梨酸的含量,灵敏度高、操作简便、准确可靠。Objective To establish an HPLC method for the determination of benzoic acid and sorbic acid in health food. Methods The Agilent ZOBAX C18 （4.6 mm×250 mm, 5 μm） column was adopted; the column temperature was 30 ℃ and the mobile phase was methanol-25 mmol ·L-1 ammonium acetate （5 ： 95）with a flow rate of 1.0 mL ·min^-1, and the detective wavelength was 230 nm. The content was calculated by external standard method. Results A good linear relationship of benzoic acid was found in the range of 1. 209-60.43μg ·mL^-1 （r=0. 9999）, and the linear range of sorbic acid was 1. 010-50. 51 μg·mL^-1 （r=0. 9998）. The average recoveries were 103.8% and 95.0%, the relative standard deviations （n=9） were 1.7% and 1.9%, respectively. Conclusion The described method can be used for content determination of benzoic acid and sorbic acid in health food, which is simple, sensitive, accurate and reliable.

关 键 词：苯甲酸 山梨酸 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]