放线菌C-411发酵液抑菌活性及活性成分  

Antibacterial activity and active ingredients of Streptomyces albus C-411

作　　者：赵娜[1] 王海丽[1] 魏少鹏[1] 姬志勤[1] 吴文君[1] 

机构地区：[1]西北农林科技大学农药研究所,陕西杨凌712100

出　　处：《植物保护学报》2013年第1期73-77,共5页Journal of Plant Protection

基　　金：国家"973"计划(2010CB126105);国家自然科学基金(30971935)

摘　　要：为了明确放线菌C-411发酵液中的抑菌活性成分和评价其在农林病害防治中的潜在应用价值,采用抑菌活性追踪法,经大孔树脂吸附、硅胶柱层析及反相高效液相色谱等方法,从C-411菌株发酵液中分离得到1个抑菌活性化合物Za-1,经ESI-MS、NMR等波谱技术鉴定为聚醚类抗生素ionophore X-206。Za-1对枯草芽胞杆菌Bacillus subtilis、蜡状芽胞杆菌Bacillus cereus、金黄色葡萄球菌Staphyloccocus aureuse和猕猴桃溃疡病菌Pseudomonas syingae有强烈的抑制作用,其最小抑菌浓度分别为0.78、1.56、3.13和6.25μg/mL。Za-1对茄子黄萎病菌Verticillium dahliae、核果炭疽病菌Drupe anthrax和苹果炭疽病菌Colletotrichum gloeosporioides菌丝生长亦有明显的抑制作用,其抑制率分别为85.10%、87.66%和88.99%。研究表明化合物Za-1有开发成农用杀菌剂的潜力。In order to explicit the inhibitory activities and evaluation the potential application of Strepto- myces albus C-411, compound Za-1 was isolated from the fermentation broth of S. albus C-411 using macroporous resin, silica gel column chromatography and RP-HPLC guided by the bioassay. The com- pound Za-1 was identified as ionophore X-206 by the means of ESI-MS, 1H-NMR, and 13C_NMR. The results of bioassay showed that Za-1 had strongly inhibitory activities against Bacillus subtilis, Bacillus ce- reus, Staphyloccocus aureuse and Pseudomonas syingae with the MIC values of 0.78, 1.56, 3.13 and 6.25 μg/mL, respectively. Additionally, Za-1 also exhibited the mycelium growth against Verticillium dahliae, Drupe anthrax and Colletotrichum gloeosporioides, with the inhibitory rates 85.10%, 87.66% and 88.99% , respectively. This implied that compounds Za-1 might be potential agricultural fungicide.

关 键 词：放线菌C-411菌株 聚醚类抗生素 IONOPHORE X-206 抑菌活性 

分 类 号：S482.28[农业科学—农药学]