番鸭乙型肝炎病毒株的分离鉴定及全基因组进化分析  

Isolation and identification,and complete-genome phylogenetic analysis of Duck hepatitis B virus strains from Muscovy duck

作　　者：刘红玉[1,2] 赵洪海[3] 陈玉环[1,2] 殷秀辰[2] 葛铭 张云[2] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所生物技术国家重点实验室,哈尔滨150001 [3]黑龙江民族职业学院,哈尔滨150066

出　　处：《黑龙江畜牧兽医》2013年第3期8-10,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：现代农业水禽产业技术体系岗位科学家专项(CARS-43-10);国家自然科学基金项目(31072132)

摘　　要：为了了解从吉林省某水禽养殖场发病番鸭的肝脏中分离到的1株鸭乙型肝炎病毒(DHBV)JL2-5株的基因型,试验采用PCR方法对其全基因组进行PCR扩增、克隆和测序。结果表明:JL2-5株全基因组序列与GenBank中14株DHBV间核苷酸的同源性为88.4%～97.7%;编码的C、S和P蛋白的氨基酸同源性分别为94.9%～98.6%、93.5%～98.2%和85.5%～97.8%,其中P蛋白的同源性最低;对DHBV ORF基因进化树的分析显示,该分离株属于中国基因型中的一个亚型。To understand the genotype of one strain （ JL2 - 5 ） of duck hepatitis B virus （DHBV） isolated from the liver of a sick Muscovy duck from a waterfowl farm, the complete genome of DHBV was amplified by PCR, and then cloned and sequenced. The results showed that the complete - genome sequences of this strain shared a homology of 88.4% to 97.7% at the nuel.eotide level with those of 14 DHBV strains from GenBank. The amino acid homologies were 94.9% to 98.6% , 93.5% to 98.2%, and 85.5% to97.8% among the encoded proteins of C, S and P respectively. Amino acid analysis showed that the protein P bad the lowest homology. The Phylogenetic analysis for the DHBV ORF region showed that this JL2 - 5 strain belonged to the subtype of Chinese genotype.

关 键 词：鸭乙型肝炎病毒 同源性 遗传进化 

分 类 号：S854.43[农业科学—临床兽医学]