以甲醇为碳源甲烷氧化细菌的高密度培养  

High-density culture of methanotrophsa with methanol as carbon source

作　　者：姜加良[1] 张帅[1] 辛嘉英[1,2] 

机构地区：[1]哈尔滨商业大学食品科学与工程重点实验室,黑龙江哈尔滨150076 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《化学工程师》2013年第2期5-8,14,共5页Chemical Engineer

基　　金：国家自然科学基金项目(20873034;21073050);校级研究生创新项目(YJSCX2011-195HSD)

摘　　要：分别考察了甲烷和甲醇对甲基弯菌IMV 3011生长的影响,并对甲醇添加方式对菌体生长的影响进行了比较。结果表明:甲醇可以代替甲烷作为碳源;当甲醇的最佳添加量为0.1%(V/V)时,间歇性添加甲醇发酵终止后的细胞密度为1.622g(dry wt).L-1,生长速率相对于甲烷提高到0.043h-1,但细胞生长的延滞期较长,为59.6254h;而相比较通过在线监测自动添加甲醇,发酵终止后的细胞密度为1.772g(dry wt).L-1,生长速率提高至0.056h-1,细胞生长的延滞期为37.1807h。说明在线监测自动添加甲醇缩短了细胞生长的延滞期即减少了发酵时间,促进了细胞的高密度生长。The effect of methane and methanol on the growth of IMV 3011 was investigated, and two kinds of methods on methanol added were compared separately. The results showed that methanol could be substituted formethane as the carbon source; With the best addition of 0.1% methanol intermittent added, the density of cell was 1.622g（dry wt）·L^-1, and the growth？rate was increased to 0.043 h^-1 relative to methane at the end of fermentation. But themeantime, a longer lag time is 59.6254 h. And in comparison to methanol intermittent added, the method of the online monitoring methanol auto-added shortened the lag time which was 37.1807h, even the period of fermenta-tion, increased the strain density which was 1.772g（dry wt）·L^-1, and the density of cell the growth rate was increased to 0.056h^-1, the high density growth of cells were promoted by online monitoring automatic adding methanol.

关 键 词：甲烷氧化菌 甲醇 在线监测 高密度培养 甲烷 

分 类 号：TQ926[轻工技术与工程—发酵工程]