对正常及大细胞性改变的肝细胞、肝癌和癌旁改变肝细胞ICM-DNA定量研究  

DNA content study of normal hepatocytes, large cell change hepatocytes, hepatocellular carcinoma and hepatocytic pericancerous change in pericancerous parenchyma with image cytometry

作　　者：陆良勇[1] 陈成伟 傅爱芬[2] 刘尚廉[3] 顾康庭 

机构地区：[1]解放军85医院 [2]上海第二医科大学病解教研室 [3]上海医科大学肿瘤医院病理科 [4]上海交通大学图象处理与模式识别研究所

出　　处：《肝脏》2000年第1期5-7,I000,共4页Chinese Hepatology

摘　　要：目的　探索正常肝细胞、大细胞性改变肝细胞、肝癌和癌旁改变肝细胞的DNA含量和形态特点。方法　采用DNA组织化学染色和图像细胞分析术。结果　10例癌旁改变(PC)组DNA指数(1.99±0.34)分别高于4例正常肝细胞(NH)组(0.98±0.12)和8例肝细胞的大细胞性改变(LCC)组(1.46±0.19)(P<0.01),DNA倍体分布与NH、LCC和肝细胞癌(HCC)组存在差异(P<0.05),核面积和核周长(74.0±52.3,63.9±33.9)低于26例HCC组(95.6±95.2,76.3±62.9)(P<0.01)。PC组中80%(8/10)为非整倍体DNA直方图表型,高于HCC组的53%(14/26),而多倍体占20%(2/10)低于HCC组26.92%(7/26)(P<0.05)。PC肝细胞核异型,核质浓缩,胞质嗜酸性或嗜碱性与HCC有过渡,置换性生长方式又与HCC相同。结论　呈片块状分布或腺瘤样排列的PC肝细胞,其异常DI、非整倍体分布和DNA直方图表型应作为癌前状态。Objective To investigate the DNA content and morphologic characteristics of normal hepatocytes (NH), large cell change hepatocytes (LCC), hepatocellular carcinoma (HCC) and the hepatocytic pericancerous change (PC) in pericancerous parenchyma. Methods By use of histochemistry staining of DNA and image cytometry.Results In 10 cases of PC group, the DNA index (DI,1.99±0.34) were higher than that in 4 cases with NH group (0.98±0.12) and 8 cases with LCC group (1.46±0.19), P <0.01, the DNA ploidy pattern was also different as compared with that in NH and LCC group ( P <0.05). The nucleus surface area and nuclear circumference (74.01±52.3, 63.89±33.9) were lower than those in 26 cases of HCC group (95.62±95.21,76.33±62.91), P <0.01. Among the PC group, 80% (8/10) were of aneuploidy pattern, more than that seen in HCC group 53% (14/26), 20%(2/10) were of polyploidy pattern, less than that in HCC group 26.92%(7/26), P <0.05. In the PC group, the hepatocytes were rich in nuclear chromatin and irregular nuclei shape, the cytoplasmic eosinophilia or basophilic showed transitional change of HCC, replaceing growth pattern showed similar with HCC.Conclusion The abnormal DNA content, DI and the ploidy pattern of PC hepatocytes which is diffuse mass like distribution and adenomatous arrangement showed precancrosis celles.

关 键 词：肝细胞癌 癌旁改良 ICM-DNA LCC HCC 

分 类 号：R735.7[医药卫生—肿瘤]