锗132对体外培养翼状胬肉成纤维细胞的增殖抑制作用观察  

An observation of antiproliferative effect of germanium-132 on cultured pterygium fibroblasts

作　　者：刘阳[1] 孙宪丽[1] 李彬[1] 王津津[2] 

机构地区：[1]北京市眼科研究所,100005 [2]首都医科大学附属北京同仁医院中心实验室

出　　处：《中华眼科杂志》2000年第4期263-266,I016,共5页Chinese Journal of Ophthalmology

摘　　要：目的　观察锗 132对体外培养翼状胬肉成纤维细胞的抗增殖作用 ,寻找辅助治疗翼状胬肉和预防翼状胬肉复发的新方法。方法　将翼状胬肉成纤维细胞行体外原代和传代培养 ;观察不同浓度锗 132及丝裂霉素C对成纤维细胞生长曲线的影响及对培养的翼状胬肉成纤维细胞增殖抑制作用和毒性作用 ;采用免疫组织化学方法检测增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)的表达。结果　锗 132可抑制体外培养的翼状胬肉成纤维细胞对PCNA的表达 ,使其生长曲线显著下移 ;对翼状胬肉成纤维细胞的增殖抑制作用呈剂量依赖性 ,且无细胞毒性反应。结论　锗Objective To detect the antiproliferation of carboxyethyl germanium sesquioxide (Ge 132) on fibroblasts of pterygium in vitro and try to find a potentially effective agent for treatment of primary pterygium and prevention of its postoperative recurrence Methods Primary culture and subculture of pterygium fibroblasts were established in vitro Different concentrations of Ge 132 (39～5 000 mg/L) or mitomycin C (3 13～4 00 mg/L, the control) were added to the fibroblast culture of the third or forth passage respectively The inhibitory effect was determined by MTT (tetrazolium bromide) method The influence of addition of Ge 132 on the growth curve of fibroblasts was observed, and the changing expression of proliferating cell nuclear antigen (PCNA) in fibroblasts was studied by immunohistochemical method Results The addition of Ge 132 in the culture caused significant inhibition of the fibroblast proliferation in dose dependent manner (625～50 000 mg/L) without cytotoxicity (IC 50 =3 000 mg/L), the marked descent of growth curve and suppression of the expression of PCNA in cultured cells ( P <0 01) Conclusion Ge 132 can inhibit the proliferation of pterygium fibroblast in vitro significantly

关 键 词：锗132 翼状胬肉 成纤维细胞 PCNA 

分 类 号：R777.33[医药卫生—眼科]