饮食限制对大鼠抗氧化能力及端粒酶活性的影响  

EFFECT OF DIETARY RESTRICTION ON ANTIOXIDANT CAPACITY AND TELOMERASE ACTIVITIES IN RATS

作　　者：胡志红[1] 杜景霞[2] 胡咏梅[1] 任爱红[1] 杨东伟[1] 王红伟[1] 

机构地区：[1]河南科技大学医学院生理教研室,洛阳471003 [2]河南科技大学医学院药理教研室,洛阳471003

出　　处：《营养学报》2013年第1期16-18,共3页Acta Nutrimenta Sinica

基　　金：洛阳市科委立项项目(No.040519)

摘　　要：目的探讨饮食限制对成年大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)及血液端粒酶活性的影响。方法 SD成年雌、雄性大鼠共48只,随机分为100%进食组(对照组)、80%进食组(限制20%组)、60%进食组(限制40%组)、50%进食组(限制50%组)4组,喂养90d。测量体重,测定血清SOD活性、MDA含量、T-AOC及端粒酶活性。结果除对照组大鼠体重增加外,限制20%组、限制40%组、限制50%组大鼠体重均明显下降(P<0.05)。各组大鼠SOD活性无明显区别;实验组大鼠限制20%组、限制40%组、限制50%组的MDA含量与对照组相比明显降低(P<0.05),限制20%组、限制40%组的T-AOC水平与对照组相比明显升高;端粒酶活性结果显示实验组与对照组相比均无明显变化。结论饮食限制可提高大鼠的抗氧化能力,但对端粒酶活性无明显影响。Objective To explore the effects of dietary restriction （DR） on serum SOD,MDA, T-AOC and blood telomerase activities in adult male and female rats. Method Forty-eight healthy adult male and female SD rats were selected and randomly divided into four groups： 100% ad libitum food intake （control）, 80% of control （20% DR）, 60% of control （40% DR）,or 50% of control （50% DR）. After 90 d, the body weight and contents of T-AOC and serum SOD, MDA, blood telomerase activities were measured. Results The body weight of rats in DR groups was reduced. The content of MDA was decreased in 20%DR, 40%DR and 50%DR groups （ P〈0.05 ） ,and the level of T-AOC was increased in 20% DR and 40%DR groups （ P〈0.05 ） .But There were no obvious differences in SOD and telomerase activities among control, 20%DR, 40%DR and 50%DR groups. Conclusion DR increases antioxidant capacity in adult rats. However, no obvious effects of DR were found on telomerase activity.

关 键 词：饮食限制 超氧化物歧化酶 丙二醛 总抗氧化能力 端粒酶 

分 类 号：R151[医药卫生—营养与食品卫生学]