明日叶查尔酮对2型糖尿病大鼠肝细胞胰岛素受体与胰岛素受体底物-2 mRNA表达的影响  

EFFECT OF CHALCONES EXTRACTED FROM ANGELICA KEISKEI ON THE mRNA EXPRESSION OF INSULIN RECEPTOR AND INSULIN RECEPTOR SUBSTRATE-2 IN HEPATOCYTES OF RATS WITH TYPE 2 DIABETES

作　　者：刘贝[1] 孙建平[2] 赵阳[1] 李蕾[1] 钟进义[1] 

机构地区：[1]青岛大学医学院营养研究所,青岛266021 [2]青岛市疾病预防控制中心,青岛266033

出　　处：《营养学报》2013年第1期44-47,共4页Acta Nutrimenta Sinica

基　　金：山东省医药卫生科技发展计划项目(No.QNW001)

摘　　要：目的研究明日叶查尔酮(Ashitabe chalcone,AC)对糖尿病大鼠肝细胞胰岛素受体(insulin receptor,InsR)和胰岛素受体底物-2(insulin receptor substrate-2,IRS-2)mRNA表达的影响。方法将用高脂饲料喂养加链尿佐菌素腹腔注射诱发的2型糖尿病大鼠随机分为糖尿病对照组和高、低剂量AC组,每组10只,均喂饲高脂饲料,分别每日经口灌胃给予明日叶查尔酮的剂量为0、30、10 mg/kg。另设一个正常对照组为正常大鼠喂饲普通饲料,实验周期4 w。用放射免疫分析法检测血清胰岛素水平、逆转录聚合酶链式反应方法检测肝细胞InsR和IRS-2 mRNA表达水平、免疫组化法测肝细胞InsR蛋白表达水平、葡萄糖氧化酶法测血糖含量。结果与正常对照组比较,糖尿病对照组空腹血糖和血清胰岛素升高,而InsR和IRS-2 mRNA表达水平降低。与糖尿病组比较,高剂量AC组空腹血糖和血清胰岛素降低,而InsR和IRS-2mRNA表达水平升高,各项差异均有显著性意义(P<0.05)。结论明日叶查尔酮可上调2型糖尿病大鼠肝细胞InsR和IRS-2mRNA表达水平,改善胰岛素抵抗状况。Objective To study the effect of chalcones extracted from Angelica keiskei （AC） on the mRNA expression of insulin receptor （InsR） and insulin receptor substrate-2 （IRS-2） in hepatocytes of rats with type 2 diabetes. Method The type 2 diabetes of rats was induced by streptozotocin with intraperitoeal injection as well as with high-fat diet feeding. The rats were randomly divided into 3 groups with 10 rats in each group, diabetic control group,high-dose AC group and low-dose AC group. All the rats were fed high-fat diet with 30 mg/kg ~ bw, 10 mg/kg ~ bw and 0 mg/kg ~ bw AC per day respectively. Another 10 normal rats fed with regular diet was used as the normal control group. After 4 weeks, serum insulin levels were evaluated by radioimmunoassay. The mRNA expression levels of InsR and IRS-2 were determined by reverse transcription polymerase chain reaction （RT-PCR）. The protein expression levels of insulin receptor in hepatocytes were detected by immunohistochemistry. Blood glucose levels were measured by glucose oxidase method. Results Compared with the rats in the normal control, the levels of blood glucose and serum insulin in the diabetic control group were increased and their InsR and IRS-2 mRNA expression levels were decreased. Compared with rats in the diabetic control gruop, the levels of blood glucose and serum insulin in high-dose AC gruop were decreased and the InsR and IRS-2 mRNA expression levels were increased. All the differences were statistically significant （P〈0.05） . Conclusion AC may up-regulate the expression levels of InsR and IRS-2 and improve insulin resistance in rats with type 2 diabetes.

关 键 词：明日叶 查尔酮 胰岛素受体 胰岛素受体底物-2 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]