土荆芥果实挥发油GC-MS分析及其生物活性研究  

GC-MS Analysis of Essential Oil from Chenopodium Ambrosioides Fruit and Study of its Biological Activities

作　　者：李植飞[1] 唐祖年[1] 戴支凯[1] 

机构地区：[1]桂林医学院,广西桂林541004

出　　处：《中国实验方剂学杂志》2013年第5期265-269,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究土荆芥果实挥发油的化学成分、抗菌活性和对HepG2细胞增殖的抑制作用。方法:采用水蒸气蒸馏法(SD)提取挥发油,通过气相色谱-质谱联用技术(GC-MS)分析土荆芥果实挥发油的化学成分,同时采用连续二倍梯度液体稀释法和转染法测定其抗菌活性,MTT法观察土荆芥果实挥发油对肝癌HepG2细胞增殖的抑制作用。结果:从土荆芥果实挥发油中共鉴定出21种成分,主要为3-异丙基-6-甲基-7-氧杂双环[4.1.0]庚-2-酮(66.93%),1-甲基-4-(1-甲基已烯基)环已烯(15.67%)及邻甲基异丙苯(12.96%);土荆芥果实挥发油对白色链株菌、表皮葡萄球菌、甲型链球菌、乙型链球菌和变形杆菌有良好的抗菌活性;对人肝癌HepG2细胞增殖有很强的抑制作用(P<0.01),孵育48,72 h后的IC50分别为0.034 1,0.069mg.L-1。结论:荆芥果实挥发油主要成分为单萜类,具有良好的抗菌作用及抑制HepG2细胞增殖作用。Objective：To investigate the chemical constituents of essential oil from Chenopodium ambrosioides fruit and study their antibacterial activities and inhibitory effects on HepG2 cell proliferation. Method： Essential oil of C.ambrosioides fruit was extracted with steam distillation （SD） and chemical constituents of the oil extrated were analyzed by Gas Chromatography-Mass Spectrometry （GC-MS）; Continuous twifold liquid gradient dilution method and transfection method were employed to determine the antibacterial activity （MIC and MBC） and MTT assay was performed to observe the inhibitory effect of the essential oil on the proliferation of HepG2 cells. Result： There were 21 ingredients identified in the the essential oil from Chenopodium ambrosioides fruit, mainly including 7-Oxabicyclo [4.1.0] heptan-2-one,6-methyl-3-（1-methyle-thyl）-（66.93%）, followed by 1-methyl-（1-methyl-alkenyl） Cyclohexene （15.67%） and o-methyl-isopropyl benzene （12.96%）. A good antibacterial effect of chenopodium ambrosioides fruit volatile oil was showed on white chain strains of bacteria, staphylococcus epidermidis, streptococcus A, streptococcus B and Proteus and a strong inhibitory effect on proliferation of human hepatoma HepG2 cells（P〈0.01）. The IC50 for 48 h and 72 h was 0.034 1 μg·mL-1 and 0.069 μg·mL-1,respectively. Conclusion： The main ingredients of the essential oil from Chenopodium ambrosioides fruit are monoterpenes, with a good antibacterial effect and inhibition of the proliferation of HepG2 cells.

关 键 词：土荆芥果实 挥发油 气相色谱-质谱联用 抗菌活性 细胞增殖 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]