大连地区454例健康体检女性人乳头瘤病毒感染调查  

Investigation on 454 women with HPV infection found by health physical examination in Dalian

作　　者：张春蕾[1] 华正宇[2] 袁小林[1] 张青[1] 杨真[1] 李小欢[1] 

机构地区：[1]大连大学附属中山医院,辽宁大连116001 [2]大连医科大学附属第一医院

出　　处：《中国妇幼保健》2013年第7期1134-1137,共4页Maternal and Child Health Care of China

摘　　要：目的:探讨大连地区健康体检女性人乳头瘤病毒(HPV)的感染情况,为宫颈癌的筛查及防治提供理论依据。方法:采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术对大连地区454例健康体检女性HPV感染进行定性检测并加以分型。结果:454例标本中,共检出HPV阳性标本58例,阳性率为12.78%;单一、双重、三重和四重基因型感染分别为46例(占79.31%)、4例(占6.90%)、6例(占10.34%)和2例(占3.45%);单纯低危型感染5例(占8.62%),单纯高危型感染46例(占79.31%),低危型与高危型混合感染7例(占12.07%)。共检出17种基因型,包括低危型HPV6、42、43和高危型HPV16、18、31、33、35、45、52、53、56、58、59、66、68、83,低危型以HPV43感染为主,高危型以HPV16、58、52感染为主。结论:健康体检女性进行HPV分型检测对HPV感染和宫颈癌的诊断、治疗具有重要意义。Objective： To explore the prevalence of human papillomavims （HPV） infection among women receiving health physical examination in Dalian, and provide theoretical basis for screening and preventing cervical cancer. Methods： DNA chip technique of PCR amplification in vitro combined with DNA reverse dot blot was used to qualitatively detect HPV infection among 454 women receiving health physical examination in Dalian. Results： Among 454 women, 58 women were found with positive HPV, the positive rate was 12.78% ; the proportions of women with single HPV infection, double HPV infection, triple HPV infection, and quadruple HPV infection were 79.31% （46 women） , 6. 90% （4 women） , 10. 34% （ 6 women） , and 3.45% （2 women） ; the proportions of women with simple low risk HPV infection and simple high low HPV infection were 8.62% （5 women） and 79.31% （46 wmnen） , respectively; 7 women were found with mixed infection of low risk HPV and high risk HPV, accounting for 12. 07%. Seventeen genotypes were screened out totally, in- chuling low risk genotypes （HPV6, 42, 43） and high risk genotypes （HPVI6, 18, 31, 33, 35, 45, 52, 53, 56, 58, 59, 66, 68, 83） ; HPV43 infection was the main type among low risk HPV genotypes, and HPV16, 58, 52 infection were the main types among high risk HPV genotypes. Conclusion： HPV genotyping detection during health physical examination among women has important significance for diagnosis and treatment of HPV infection and cervical cancer.

关 键 词：人乳头瘤病毒 基因分型 宫颈癌 DNA芯片技术 

分 类 号：R737.33[医药卫生—肿瘤]