蚯蚓体内过氧化物还原蛋白PRDX基因对土壤PAHs污染胁迫的转录响应  

Impact of PAHs on the Expression of PRDX in Earthworm(Eisenia fetida)

作　　者：林康丽[1,2] 郑森林[3] 宋玉芳[1] 邱晓燕[4] 薛文玲[3] 

机构地区：[1]中国科学院沈阳应用生态研究所中国科学院污染生态与环境工程重点实验室,沈阳110016 [2]中国科学院大学,北京100049 [3]国家海洋局第三海洋研究所,厦门361005 [4]集美大学生物工程学院,厦门361021

出　　处：《环境科学》2013年第3期1204-1210,共7页Environmental Science

基　　金：国家自然科学基金项目(20977094;40930739);国家海洋公益性行业科研专项(200905011;201005016);福建省自然科学基金项目(2009J05077)

摘　　要：在前期建立的苯并[a]芘(benzo[a]pyrene,BaP)诱导上调表达的蚯蚓cDNA文库中,发现一种过氧化物还原蛋白peroxiredoxin6(PRDX6)基因片段,并提交到GenBank.Blastx比对与基因进化分析结果表明,该序列与已知PRDX6的最高相似率为86%,期望值为4E-30.序列分析表明,该序列具有PRDX6基因含Cys编码的一个特征motif,表明该序列属于PRDX6基因.为进一步验证蚯蚓PRDX6基因对多环芳烃污染的响应情况,使用OECD推荐方法,进行了土壤菲、芘、荧蒽和苯并芘污染对蚯蚓的毒性响应的试验(周期28 d),并采用定量PCR方法,检测了各实验组蚯蚓PRDX6基因的表达差异.结果发现,1.0 mg·kg-1的芘和苯并[a]芘均可显著上调蚯蚓PRDX6基因的表达,表明PRDX6基因可作为土壤污染引起蚯蚓抗氧化应激检测的分子标记.A peroxiredoxin6 （PRDX6）-like gene segment has been found in up-regulated eDNA libraries of earthworm Eisenia fetida exposed to benzo[a] pyrene（BaP）. Analysis with basic local alignment search tool （BLAST） and phylogenetic analysis revealed that the gene segment contains a characteristic motif which was encoded by Cys, illustrating that this gene segment belongs to PRDX. To verify the response of PRDX in E. feitida exposed to poly aromatic hydrocarbons （PAHs） , four-week pollution experiments were conducted following the methods recommended by the Organization for Economic Co-operation and Development （OECD）. The results showed that the expression of PRDX was up-regulated in earthworm exposed to 1.0 mg-kg -1 Pyrene and BaP. It was indicated that PRDX in E. fetida was the potential molecular biomarkers of antioxidative stress caused by soil contamination.

关 键 词：蚯蚓 PRDX6 PAHS 差异表达 生物标记物 

分 类 号：X171.5[环境科学与工程—环境科学]