一株产脂肪酶芽孢杆菌的鉴定及其脂肪酶基因LIP的克隆分析  

Identifying a lipase-producing Bacillus strain and cloning its LIP gene

作　　者：刘生峰[1,2] 肖进文[1,2] 周蓓莉[1,3] 杨俊[1,2] 聂福平[1,2] 王昱[1,2] 周庆[1,2] 李应国[1,2] 

机构地区：[1]重庆出入境检验检疫局,重庆400020 [2]重庆市进出口食品安全工程研究中心,重庆400020 [3]西南大学,重庆400715

出　　处：《食品工业科技》2013年第5期147-150,共4页Science and Technology of Food Industry

基　　金：重庆市科技攻关项目(2011AC1056;2011AC1057);国家质检总局公益项目(201010045)

摘　　要：依据形态学和生理生化特征,将一株产脂肪酶芽孢杆菌BL03初步鉴定为地衣芽孢杆菌(Bacillus licheniformis)。提取该菌核酸,PCR扩增出地衣芽孢杆菌脂肪酶基因LIP,克隆到pMD19-T载体后进行PCR鉴定及序列测定和生物信息学分析,结果表明该菌LIP基因片段完整,开放阅读框为615bp,编码204个氨基酸,与已发表地衣芽孢杆菌脂肪酶基因(GeneID:3098655)相比,核酸序列相似为99.67%,推导编码氨基酸序列相似性为99.02%,该基因的获得为其异源表达及相关研究奠定了基础。Bacillus strain BL03 was identified as Bacillus lichenifermis based on its morphological, physiology and biochemistry characteristics.The LIP gene encoding lipase was amplified from B.Iicheniformis BL03 and cloned to vector pMD19-T.Nucleotide sequence analysis of the LIP gene revealed that the coding region was formed of one complete ORF including 6].5 base pairs encoding 204 amino acids. The cloned gene was 99.6？% similar with B.licheniformis LIP gene（GenelD：3098655） and the putative amino acid sequence was 99.02% similar respectively. The cloned gene laid foundation for the heterologous expression and relevant researches on it.

关 键 词：地衣芽孢杆菌 脂肪酶基因 克隆 序列分析 

分 类 号：Q937.97[生物学—微生物学]