氟化物对家蚕血液羧酸酯酶及全酯酶活性的影响  

Influence of fluoride on activity of carboxylesterase and esterase in hemolymph of Bombyx mori

作　　者：米智[1] 阮成龙[1] 李姣蓉[1] 付巧娟[1] 杜文华[1] 李冠楠[1] 隆耀航[1] 朱勇[1] 

机构地区：[1]西南大学生物技术学院,重庆400716

出　　处：《生态学报》2013年第4期1134-1141,共8页Acta Ecologica Sinica

基　　金：重庆市重大攻关项目(CSTC;2009AA1024)

摘　　要：为了探讨氟化物对家蚕代谢机制的影响,以家蚕耐氟品种T6和氟化物敏感品种734为研究对象,从5龄起蚕开始分别添食50、100、200、400mg/kg NaF溶液浸泡后的新鲜桑叶,检测家蚕血液中羧酸酯酶(CarE),全酯酶活性的变化。结果表明,734、T6添氟组的CarE活性分别是对照组的73%—88%和72%—81%,734两个低浓度添氟组的CarE活性与对照组和两个高浓度添氟组的差异极显著(P<0.01),T6各处理组之间的差异不显著。734、T6添氟组的全酯酶活性分别是对照组的89%—97%和73%—92%,734各处理组之间的差异不显著,T6对照组的全酯酶活性仅与最高浓度添氟组差异极显著(P<0.01)。说明氟化物对家蚕血液CarE和全酯酶活性具有一定的抑制作用。We explored the metabolic mechanism of silkworm（Bombyx mori） to NaF by using the fluoride-tolerant silkworm variety named T6 and the fluoride-sensitive variety named 734 as materials.We fed the silkworm larvae from the first day of fifth instar with mulberry leaves soaked in 50,100,200 and 400 mg/kg NaF solutions respectively.Then we examined the activities of CarE and esterase in hemolymph.Results showed that the activity of CarE was 73% to 88% in 734 strain and 72% to 81% in T6 strain compared with control groups,respectively.The CarE activity of two low concentration NaF-treated 734 groups showed extremely significant differences（P0.01） with control group and two high concentration NaF-treated groups,while the differences among T6′s all treatment groups were not significant.The esterase activity of NaF-treated groups was 89% to 97% in 734 strain and 73% to 92% in T6 strain compared with control groups,respectively.The esterase activity among all treatment groups in 734 strain show no significant differences.The esterase activity of the highest concentrations NaF-treated group showed extremely significant differences（P0.01） compared with control group.These results suggest that the fluoride has certain inhibition to the activity of CarE and esterase in hemolymph of silkworm.

关 键 词：家蚕 氟化物 血液 羧酸酯酶 全酯酶 

分 类 号：X174[环境科学与工程—环境科学]