HLA-Ⅰ类抗原及内质网分子伴侣表达下调与新疆哈萨克族食管鳞癌的关系  

Association of down-regulated expression of HLA-Ⅰ antigen and endoplasmic reticulum molecular chaperones with clinicopathological features of esophageal squamous cell carcinoma in Xinjiang Kazakh patients

作　　者：郑峰[1] 李德生[1] 居来提.安尼瓦尔 张力为[1] 伊力亚尔.夏合丁 

机构地区：[1]新疆医科大学附属医院胸外科,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《世界华人消化杂志》2013年第5期386-391,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨人类白细胞抗原Ⅰ类分子(human leukocyte antigen classⅠ,HLA-Ⅰ)类抗原及内质网分子伴侣在新疆哈萨克族食管癌中的表达水平及其与临床病理特征之间的关系.方法:用免疫组织化学法检测50例新疆哈萨克族食管鳞癌、癌旁石蜡包埋组织中HLA-Ⅰ类分子和内质网分子伴侣中的表达水平,同时分析其蛋白表达下调和缺失与临床病理特征的关系.结果:在食管癌组织中,HLA-Ⅰ类抗原和内质网分子伴侣钙连接蛋白(CNX)、TAP相关蛋白(Tapasin)、蛋白质二硫异构体(Erp57)的表达下调和缺失率分别为24%/68%、20%/48%、20%/52%、16%/32%,在癌旁组织中的表达下调和缺失率为4%/0%、10%/2%、8%/2%、16%/2%.食管癌组织中HLA-Ⅰ分子及内质网分子伴侣较癌旁组织中有明显的表达下调或缺失,其差异均有统计学意义(P<0.05).在临床病理特征中,HLA-Ⅰ与肿瘤分化、淋巴结转移、肿瘤浸润深度密切相关;CNX与肿瘤分化、血管侵袭、淋巴结转移、肿瘤浸润深度密切相关;Erp57与淋巴结转移、血管侵袭、肿瘤浸润深度密切相关;Tapasin与肿瘤分化、肿瘤浸润深度密切相关,表达差异均有统计学意义(P<0.05).结论:新疆哈萨克族食管癌中有明显的HLA-I类抗原及内质网分子伴侣的表达下调或缺失,这与哈萨克族食管癌的发生及肿瘤的临床病理特征密切相关.AIM： To investigate the expression of human leukocyte antigen class-I （HLA-I ） and endo plasmic reticulum molecular chaperones and to analyze their relationship with clinicopathologi- cal features of esophageal squamous cell carci- noma （ESCC） in Kazakh patients. METHODS： The expression of HLA-I and endo-plasmic reticulum molecular chaperones was as- sessed by immunohistochemistry in 50 paraffinembedded ESCC specimens and tumor-adjacent normal mucosal specimens. The associations of expression of HLA-I and endoplasmic reticulum molecular chaperones with clinicopathological features of ESCC were analyzed.RESULTS： The rates of down-regulation or loss of expression of HLA-I protein and en- doplasmic reticulum molecular chaperones （CNX, Tapasin, and Erp57） were 24%/68%, 20%/48%, 20%/52% and 16%/32% in ESCC, and %/0%,10%/2%,8%/2% and 16%/2% in tumor adjacent normal mucosal specimens. The posi- tive rates of HLA- I and endoplasmic reticulum molecular chaperones significantly decreased in ESCC compared with control tissue （P 〈 0.05）. HLA- I expression was significantly correlated with pathological grade, lymph node metastasis and depth of invasion. Erp57 expression was significantly correlated with vascular invasion, lymph node metastasis and depth of invasion. Tapasin expression was significantly correlated with lymph node metastasis and depth of in- vasion. Calnexin expression was significantly correlated with all above clinicopathological pa- rameters （all P 〈 0.05）.CONCLUSION： Expression of HLA- I and endoplasmic reticulum molecular chaperones was significantly down-regulated in ESCC in Xinjiang Kazakh patients. The abnormal expression of HLA-I and endoplasmic reticulum molecular chaperones is closely related with the occurrence and clinicopathological features of ESCC.

关 键 词：食管鳞状上皮细胞癌 HLA-I类抗原 内质网分子伴侣 

分 类 号：R735.1[医药卫生—肿瘤]