Bmi-1和Mel-18基因在大肠癌组织中的表达及意义  

Significance of expression of Bmi-1 and Mel-18 in colorectal carcinoma

作　　者：邹艳芳[1] 田永[1] 徐峰[1] 

机构地区：[1]郑州大学第一附属医院消化内科,河南省郑州市450052

出　　处：《世界华人消化杂志》2013年第5期397-402,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨Bmi-1和Mel-18基因在大肠癌中的表达及其临床意义.方法:收集我院68例大肠癌组织标本,68例癌旁正常组织作为对照.利用免疫组织化学的方法检测大肠癌及正常组织中的Bmi-1基因和Mel-18基因的表达,并结合临床资料,对该两种基因的表达与大肠癌患者临床表现的相关性进行分析.结果:(1)Bmi-l在大肠癌组织中的表达明显高于正常组织(73.5%vs23.5%,P<0.05),且与大肠癌的侵袭深度、淋巴结转移及临床分期有关(P<0.05);(2)Mel-18基因在大肠癌组织中的表达明显低于正常组织(41.2%vs66.2%,P<0.05),且与大肠癌的淋巴结转移及临床分期呈负相关(P<0.05);(3)相关性分析发现,Bmi-1和Mel-18基因在大肠癌组织中的表达呈负相关(r=-0.545,P<0.05).结论:Bmi-1和Mel-18基因与大肠癌的发展、转移关系密切,检测Bmi-1和Mel-18对大肠癌的诊断及判断预后可能有重要意义.AIM： To investigate the relationship between the expression of Bmi-1 and Mel-18 and clinicopathological features of colorectal carcinoma. METHODS： Sixty-eight colorectal cancer sam- ples and matched tumor-adjacent normal tissue samples were collected to detect the expression of Bmi-1 and Mel-18 using immunohistochemistry. The relationship between the abnormal expression of Bmi-1 and Mel-18 and the clinicopathological features of colorectal carcinoma was analyzed. RE SU ITS： The positive rate of Bmi-1 expression was significantly higher in colorectal carcinoma than in normal colon tissue （P 〈 0.05）, and expression of Bmi-1 was correlated with the depth of bowel wall invasion, lymph node metastasis and Dukes stage （all P 〈 0.05）. The positive rate of Mel-18 expression was significantly lower incolorectal carcinoma than in normal colon tissue （P 〈 0.05）, and expression of Mel-18 was negatively related to lymph node metastasis and Dukes stage （both P 〈 0：05）. There was a negative correlation between the expression of Bmi-1 and Mel-18 in colorectal carcinoma （r = -0.335, P 〈 0.05）. CONCLUSION： The expression of Bmi-1 and Mel-18 proteins correlates with the progression, metastasis and prognosis of colorectal cancer. Combined detection of Bmi-1 and Mel-18 pro- tein expression may be helpful to the diagnosis and evaluation of the malignancy of colorectal carcinoma.

关 键 词：结直肠肿瘤 BMI-1 Mel-18 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]