聚乙二醇滴眼液对拉坦前列腺素眼表毒性的中和作用  

Neutralization of polyethylene glycol on ocular surface toxicity induced by benzalkonium chloride-preservedlatanoprost

作　　者：余芬芬[1] 钟毅敏[1] 李媚[1] 毛真[1] 郭歆星[1] 杨莎莎[1] 曹丹[1] 肖辉[1] 刘杏[1] 

机构地区：[1]中山大学中山眼科中心眼科学国家重点实验室,广州 510060

出　　处：《中华实验眼科杂志》2013年第3期227-232,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：广东省自然科学基金项目（$2011010006046）;眼科学国家重点实验室创新基金项目（2010C03）

摘　　要：背景减少局部抗青光眼药物的眼表毒性、提高患者对药物治疗的依从性已经成为青光眼药物治疗过程中亟待解决的问题。聚乙二醇是一种新型的人工泪液，其是否可以减轻抗青光眼药物局部应用后的眼表毒性尚待研究。目的探讨聚乙二醇对拉坦前列腺素滴眼液中防腐剂苯扎氯胺所致眼表毒性的影响。方法采用随机数字表法将30只新西兰大白兔分为3个组，每组10只，正常对照组兔眼不给予任何干预，PBS处理组给予质量分数0．005％拉坦前列腺素联合PBS溶液连续点眼60d，聚乙二醇处理组给予0．005％拉坦前列腺素联合聚乙二醇滴眼液点眼60d。分别于用药前及用药后第31天和第61天行兔眼基础泪液分泌试验、角膜荧光素染色评分、角膜结膜虎红染色评分和结膜印迹细胞学检查，并于第61天获取角膜及球结膜组织标本行扫描电子显微镜和透射电子显微镜观察。结果对照组、PBS处理组及聚乙二醇处理组在实验第31天和第6l天的角膜荧光素染色评分差异均有统计学意义（H=25．265、29．426，P〈0．001）；3个组在实验第31天和第61天角膜球结膜虎红染色评分差异均有统计学意义（H=26．167、29．212，P〈0．001）；各组不同时间点基础泪液分泌量的差异有统计学意义（F女；镕目=4．746，P〈0．05），各组不同时间点球结膜杯状细胞密度的差异有统计学意义（F=18．055，P〈0．001）。与对照组比较，PBS处理组第6l天角膜荧光素染色评分[0（0，1．0）VS．3（2．0，3．0）]及虎红染色评分[0（0，1）VS．2（2，3）]均明显升高，差异均有统计学意义（P〈0．001）；PBS处理组第61天基础泪液分泌量和结膜上皮杯状细胞密度均明显低于对照组[（5．18＋0．46）mmVS．（9．43±O．57）mm；（53．04~2．98）个／高倍视野VS．（87．73±2．34）个／高倍视野]，差异均有统计学意义（P〈0．01）Background Reducing ocular surface toxicity induced by topical antiglaucoma medications,and thus improving the therapeutic compliance of glaueomatous patients to treating drug become an urgent issue in the long-term antiglaucomatous therapies. Whether polyethylene glycol, a new type of artificial tear, can play a protective role from ocular surface toxicity induced by topical antiglaucoma medications remains further study. ObjectiveThe present study was to investigate the protective effect of polyethylene glycol on ocular surface toxicity induced by prolonged use of benzalkonium chloride-preserved latanoprost eye drops. Methods Thirty adult female New Zealand rabbits were randomly divided into normal control group, phosphate buffer solution （PBS） group and polyethylene glycol eye drops group. PBS with 0. 005% latanoprost was topically administered for consequent 60 days in the PBS group, and 0. 005% latanoprost with polyethylene glycol eye drops was used at the same way in the polyethylene glycol group. Schirmer I test,fluorescein and rose bengal staining of cornea and conjunctiva,conjunctiva impression cytology specimens collecting were performed before experiment as well as 31 and 61 days after administration of drug. Histomorphological and uhrastructural changes of cornea and conjunctiva were observed under the light microscopy, scanning and transmission electron microscopy on day 61. Results The score of corneal fluorescine staining was significantly different among the control group,PBS group and polyethylene glycol group （H= 25. 265 ,P〈0. 001 ;H=29. 426,P〈0. 001 ） on day 31 and day 61. Significant difference also was found in the score of rose bengal staining of cornea and conjunctiva （ H= 26. 167, P〈0. 001 ;H= 29. 212 ,P〈O. 001 ） , Schirmer I test value （ F=4. 746 ,P〈0.05 ） and conjunctival goblet cell density （ F= 18. 055 ,P〈0. 001 ）. Compared with the control group, cornealfluorescein scores [0（0,1.0） vs. 3 （2.0,3.0）1 and rose bengal scores [0（0, 1） vs. 2（2,3

关 键 词：聚乙二醇 拉坦前列腺素 苯扎氯胺 眼表毒性 

分 类 号：R77[医药卫生—眼科] R9[医药卫生—临床医学]