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出 处:《华西口腔医学杂志》2000年第5期332-335,共4页West China Journal of Stomatology
摘 要:目的 :评价 2 %戊二醛浸泡、喷涂 ,5 %艾力克浸泡、喷涂 ,臭氧柜处理对口腔印模和石膏模型上的表皮葡萄球菌、血液链球菌和枯草杆菌芽孢的消毒效果。方法 :将无菌的印模和石膏模型平均分成 3组 ,每组印模和石膏模型表面分别涂表皮葡萄球菌悬液、血液链球菌悬液和枯草杆菌芽孢菌悬液 ,并设立同期空白对照组。采用 2 %戊二醛浸泡、喷涂与 5 %艾力克浸泡、喷涂分别对染菌印模进行消毒处理。臭氧柜对石膏模型进行消毒处理。其后的不同时间在印模与石膏模型表面一定位置取样、接种、培养并进行CFU计数 ,计算各种消毒方式的抑菌率。根据抑菌率大小评价消毒效果。结果 :5种消毒方式对表皮葡萄球菌、血液链球菌消毒效能无显著差异 ,可达 98 3%~ 10 0 %的抑菌率。对枯草杆菌芽孢 ,则表现出效能差异 ,即浸泡法强于喷涂法 ,更强于臭氧柜法。结论 :2 %戊二醛浸泡、喷涂与 5 %艾力克浸泡、喷涂 ,均为有效的印模消毒方法 ,而臭氧消毒法可作为石膏模型的消毒方法。Objective: In this study, the disinfecting efficiency of five disinfecting methods to three bacterial: Staphylococcus epidermidis, Streptococcus sanguis and Bacillus subtilis were evaluated. Methods: Germ free impressions and gypsum casts were divided into three teams contained 16 impressions and 5 gypsum casts for each one. Each team was smeared with each one of the three bacterial solutions. Then four disinfecting methods were administrated on these impressions separately, 2% glutaraldehyde immersion, 2%glutaraldehyde spray, 5% Eric immersion, 5% Eric spray. And ozone treatment was administrated on gypsum casts. Control teams were set up. After the treatment the impressions and gypsum casts were sampled at standard sites. The colonies were counted after culture and were used to deduce the germicidal ratio as the standard of disinfecting efficiency. Results: There was no difference between the efficiencies of five disinfecting methods for Streptococcus sanguis and Staphylococcus epidermidis. But for B. subtilis, the immersion methods have the highest efficiency among the methods and the ozone treatment has the least efficiency. Conclusion: 2% glutaraldehyde immersion, spray, 5% Eric immersion, spray are all effective disinfecting methods for impressions and ozone treatment is an effective method in disinfecting the gypsum casts.
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