运动训练对大鼠局灶脑梗死后脑内LINGO-1的表达及其对神经功能的影响  

Effects of exercise training on expressions of LINGO-1 in the brain of rats and neural function after focal cerebral infarction

作　　者：黄浩然[1] 李光勤[1] 付敏[1] 赖智勇[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《重庆医科大学学报》2013年第2期134-137,共4页Journal of Chongqing Medical University

摘　　要：目的:观察大鼠运动训练后脑内LINGO-1表达的变化,从而揭示运动训练促进脑卒中后神经功能恢复的可能分子机制。方法:采用线栓法制备大脑中动脉闭塞(middle cerebralartery occlusion,MCAO)模型,随机将大鼠分为假手术组、模型组及运动训练组,选取3、7、14 d 3个时间点观察,运动训练采用跑台跑步方法。运用Longa评分进行神经功能评价,RT-PCR进行LINGO-1 mRNA检测,免疫组织化学观察LINGO-1蛋白水平变化。结果:假手术组无明显神经功能缺损,Longa评分0分,其LINGO-1 mRNA及蛋白水平有少量阳性表达。模型组LINGO-1表达3 d为高峰,后逐渐降低(P<0.05)。运动训练组与模型组相比,在3 d及7 d时间点Longa评分、mRNA及蛋白水平表达无明显差异(P>0.05),在14 d时间点Longa评分、LINGO-1 mRNA及蛋白水平均有下降(P<0.05)。结论:局灶性脑梗死后大鼠脑内LINGO-1水平明显上调,适当时间的运动训练可降低LINGO-1的表达水平及神经功能评分,这可能是运动训练促进脑梗死后神经功能恢复的分子机制之一。Objective：To observe the expressions of LINGO-1 in the brain of rats after exercise training and to explore the possible molecular mechanism of exercise training promoting neurological functional recovery after stroke. Methods ： Middle cerebral artery occlusion（MCAO） model was made and rats were randomly divided into sham operation group, model group and exercise training group. Observation was made at 3,7,14 d after MCAO. Training method was treadmill exercise. Neurological functional recovery was evaluated by Longa＇s score;expressions of LINGO-1 mRNA and protein were detected by imunohistochemistry and RT-PCR. Results： Sham group had no obvious neurological deficiency（Longa score was 0） but small amount of mRNA and protein expressions of LIN- GO-1. mRNA and protein expressions of LINGO-1 in model group were reached the peak at 3 d after MCAO and decreased gradual- ly later（ P〈O.05 ）. There was no difference in mRNA and protein expressions of LINGO-1 between exercise training group and model group at 3,7 d after MCAO（P〉0.05）. Longa score and mRNA and protein expressions of LINGO-I were decreased at 14 d after MCAO in exercise training group compared with those in model group （P〈0.05）. Conclusions：Expressions of LINGO-1 in the ischemic brain are increased significantly. Decreased neurological score and LINGO-I mRNA and protein expressions induced by proper exercise training might be one of the molecular mechanisms of neural function recovery after acute cerebral infarction.

关 键 词：脑梗死 LINGO-1 运动训练 

分 类 号：R743.3[医药卫生—神经病学与精神病学]