成纤维细胞生长因子10单克隆抗体对豚鼠银屑病样模型的作用研究  

Effect of a monoclonal antibody against fibroblast growth factor 10 in a guinea pig model of psoriasis-like skin inflammation

作　　者：梅向林[1] 夏建新[1] 牟妍[1] 王敬医[1] 李雪[1] 朱文静[1] 李福秋[1] 金仙花[1] 于凯[1] 

机构地区：[1]吉林大学第二医院皮肤科,长春130041

出　　处：《中华皮肤科杂志》2013年第3期193-195,共3页Chinese Journal of Dermatology

基　　金：吉林省科技发展计划（201109589）

摘　　要：目的探讨成纤维细胞生长因子（FGFIO）单克隆抗体局部外用对银屑病豚鼠模型的治疗作用。方法应用5％盐酸普萘洛尔搽剂外涂豚鼠耳背部皮肤，诱导银屑病样动物模型。分别设置空白组、模型组、丁酸氢化可的松治疗组、FGFIO抗体高（0．188g／L）、中（0．094g／L）、低（0．063g／L）剂量治疗组。治疗2周后观察银屑病样模型的病理变化。采用SPSS13．0统计软件进行统计学分析，Baker评分比较应用秩和检验，单一核细胞计数及表皮厚度比较应用方差分析（ANOVA），多个样本均数的两两比较应用最少显著差法（LSD）。结果各治疗组上述各项检测指标均低于模型组（均P〈0．05）。FGFIO抗体高剂量组炎细胞计数与空白组差异无统计学意义（t=0．77，P〉0．05），其余各治疗组炎细胞计数高于空白组及FGF10抗体高剂量组（均P〈0．05）。FGF10抗体各治疗组表皮厚度均高于丁酸氢化可的松治疗组，差异均有统计学意义（均P〈0．05）。FGF10抗体各治疗组组间表皮厚度比较，差异无统计学意义（均P〉0．05）。结论FGF10单克隆抗体对银屑病样豚鼠模型中异常病理改变有显著调节作用，能影响角质形成细胞增殖分裂，还能显著抑制银屑病模型中炎症反应。Objective To evaluate the therapeutic effect of local application of an anti-fibroblast growth factor 10 （FGFIO） monoclonal antibody （MoAb） on a guinea pig model of psoriasis-like skin inflammation. Methods A model of psoriasis-like skin inflammation was established by applying 5% propranolol hydrochloride emulsion to the dorsal skin of ears of 45 guinea pigs, which were then classified into 5 groups： model group receiving no treatment, hydrocortisone butyrate group, high-, medium- and low-dose MoAb groups receiving topical treatment with hydroeortisone butyrate, anti-FGF10 MoAb of 0.188 g/L, 0.094 g/L and 0.063 g/L, respectively, twice daily. Nine guinea pigs receiving no propranolol challenge nor treatment served as the blank control group. The response of guinea pigs to propranolol hydrochloride emulsion and therapeutic agents, such as scratch behavior, hair status and skin status, was observed and recorded. After two weeks of treatment, all the guinea pigs were sacrificed, and skin samples were resected from the ears followed by the evaluation of Baker score, measurement of epidermal thickness, and enumeration of mononuclear cells. SPSS 13.0 software was used for data processing. Baker score was compared by rank sum test, mononuclear cell count and epidermal thickness by analysis of variance. Pairwise comparisons were carried out by the least significant difference （LSD） procedure. Results The number of mononuclear ceils was similar between the high-dose MoAb group and blank control group （t = 0.77, P 〉 0.05）, but higher in the other treatment groups than in the high-dose MoAb group and blank control group （all P 〈 0.05）. Increased epidermal thickness was observed in the three MoAb groups compared with the hydrocortisone butyrate group （all P 〈 0.05）, while there was no statistical difference between the three MoAb groups （all P 〉 0.05）. Conclusions The anti-FGF10 MoAb obviously attenuates the pathological changes, affects the proliferation of keratinocytes, and markedly

关 键 词：成纤维细胞生长因子10 银屑病样模型 单克隆抗体 豚鼠模型 丁酸氢化可的松 统计学分析 角质形成细胞增殖 盐酸普萘洛尔 

分 类 号：R-332[医药卫生] R758