缺氧培养骨髓内皮祖细胞移植对缺血性心脏病的治疗作用  

The Therapeutic Effect of Hypoxic Preconditioning of Endothelial Progenitor Cell Transplantation on Ischemia Heart Disease

作　　者：简锴陶[1] 王联群[1] 王强[1] 郑宝荣[1] Joseph Y.Woo 刘建实[1] 

机构地区：[1]天津市胸科医院,300051 [2]宾夕法尼亚大学附属医院

出　　处：《天津医药》2013年第2期145-147,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(项目编号:12JCYBJC33100)

摘　　要：目的探讨缺氧培养的骨髓内皮祖细胞心肌内移植治疗缺血性心脏病的效果。方法从8周龄同种系成年雄性Wistar大鼠长骨中获取骨髓内皮祖细胞,常规培养4d后,1%O2+5%CO2+94%N2培养3d。将26只8周龄同种系成年雄性Wistar大鼠随机分为对照组(n=8)、常规组(n=9)和缺氧组(n=9),建立急性心肌梗死动物模型。在心肌梗死边缘5个不同区域心肌内分别注射PBS溶液200μL,内皮祖细胞2×106个,缺氧培养内皮祖细胞2×106个,4周后应用压力-容量曲线分析评估实验动物血流动力学和心脏功能变化。结果缺氧培养骨髓内皮祖细胞心肌内移植4周后,缺氧组与对照组相比较,舒张末容积、收缩末容积、心搏量、射血分数以及每分心输出量改善明显,差异均有统计学意义;缺氧组与常规组相比,收缩末容积减小,心搏量、射血分数及每分心输出量提高,差异均有统计学意义;常规组与对照组比较,心搏量、射血分数和每分心输出量无明显改善,差异无统计学意义。结论缺氧培养大鼠骨髓内皮祖细胞较常规培养细胞对缺血性心脏病的治疗效果更加明显,限制了心室重构,改善了梗死后整体的心脏功能。Objective To investigate the therapeutic effectiveness of transplantating hypoxic preconditioning endo- thelial progenitor cells （HEPCs） on acute myocardial infarction. Methods Bone marrow endothelial progenitor cells （EPCs） were isolated from 8-week syngeneic adult male Wistar rats. After normoxic culture for 4 days, EPCs were cultured in 1% 05＋ 5% CO2＋94% N2 for 3 days. Then, 26 8-week syngeneic adult male Wistar rats were randomized into 3 groups： control group （n=8）, EPCs group （n=9） and HEPCs group （n=9）. The acute myocardial infarction animal model was set up. Rats received 5-points peri-infarct intramyocardial injections of PBS 200 μL 2×10^6 EPCs and 2×10^6 HEPCs. After 4 weeks, the pres- sure-volume （P-V） curve analysis was used to evaluate the haemodynamies parameters and cardiac function. Results After 4 weeks of transplantation, P-V loop analysis showed that there were significant differences in end diastolic volume, end sys- tolic volume, stroke volume,ejection fraction（EF） and cardiac output between HEPCs and control groups. There were signifi- cant differences in end systolic volume, stroke volume and cardiac output between HEPCs and EPCs groups. There were no significant differences in stoke volume and cardiac output between EPCs and control groups. Conclusion Transplantation of HEPCs limited left ventrieular remodeling and improved cardiac function entirely after acute myocardial infarction.

关 键 词：干细胞 内皮细胞 骨髓细胞 干细胞移植 心肌梗死 心脏功能试验 大鼠 Wistar 

分 类 号：R733.3[医药卫生—肿瘤]