DNA酶Ⅰ抑制细胞聚集降低骨髓间充质干细胞静脉注射发生急性肺栓塞的风险  

DNase I Can Reduce Acute Pulmonary Embolism Risk after Intravenous Injection of Bone Marrow Mesenchymal Stem Cells by Inhibiting Cell Aggregation

作　　者：时炳正[1] 徐志媛[1] 杜晓岩[1] 杜建宇[1] 范龙坤[2] 毕焕京[3] 廖立[3] 金岩[3] 

机构地区：[1]佳木斯大学口腔医学院,黑龙江佳木斯154007 [2]沧州市中心医院口腔颌面外科 [3]第四军医大学口腔医学院组织工程中心

出　　处：《口腔医学研究》2013年第2期111-116,共6页Journal of Oral Science Research

基　　金：国家基础研究项目(973项目)(2010CB944800、2011CB964700);黑龙江省研究生创新科研项目(YJSCX2012-359HLJ);佳木斯大学重大项目培育基金项目(SZP2001-001)

摘　　要：目的:探索DNA酶Ⅰ处理后骨髓间充质干细胞降低急性肺栓塞的发生。方法:体外培养小鼠骨髓间充质干细胞。C57小鼠分PBS注射组、10U/mL DNaseⅠ-PBS注射组、正常BMSCs注射组、DNaseⅠ处理后BMSCs注射组。PBS注射组与正常BMSCs注射组尾静脉注射PBS或临界致死剂量的BMSCs,脏器组织学检测,PBS注射组对照,观察急性死亡率;比较正常BMSCs与DNaseⅠ处理后BMSCs体外增殖、分化能力和悬液中细胞聚集状态;DNaseⅠ处理后BMSCs注射组注射DNaseⅠ处理后3×106个BMSCs 5ml/kg观察急性死亡率。结果:PBS注射组相比正常BMSCs注射组急性死亡率为60%、心肺异常、肺血管内异染物质,急性肺栓塞;DNaseⅠ预处理不影响BMSCs体外增殖分化能力;DNaseⅠ处理BMSCs较正常BMSCs聚集成团细胞比例显著下降。DNaseⅠ处理后BMSCs注射组的急性死亡率为23.3%,与正常BMSCs注射组比较差异显著。结论:DNaseⅠ预处理不影响BM-SCs的生物学特性并能抑制单细胞聚集降低静脉注射BMSCs所导致的急性肺栓塞。Objective： To investigate a novel method to reduce the occurrence of pulmonary embolism after BMSCs injection by using DNase I pretreatment. Methods： Mouse BMSCs were isolated, cultured and purified to the 3rd generation in vitro. C57BL/6 mice were divided into 4 groups： PBS injection group, 10U/ml DNase I-PBS injec- tion group, BMSCs injection group and DNase I pretreated BMSCs injection group. Acute death rate were calculated and different organs of dead mice were examined by histological analysis. Proliferation and differentiation ability a- long with aggregation status in cell suspension of BMSCs before and after DNase I treatment were detected in vitro. Result： The acute death rate of BMSCs injection group was 40% lower than PBS injection group, and histological examination revealed cardiopneumatic abnormality. Some deeply stained masses were found in pulmonic vessel. DNase I pretreatment did not affect the proliferation and differentiation ability of BMMSCs in vitro, but the propor- tion of aggregated cells declined significantly. The acute death rate of DNase I pretreated BMSCs injection group was 23.3%, which was significantly lower than BMSCs injection group. Conclution： DNase I pretreatment would not af- fect the biological character of BMSCs but could inhibit single cell aggregation.

关 键 词：骨髓间充质干细胞 细胞鉴定 DNASE Ⅰ 注射风险 

分 类 号：R965[医药卫生—药理学]