N-甲基亚硝基脲的制备及其诱发膀胱肿瘤的作用  

Synthesis of N-methyl-N-nitrosourea (MNU) and development of experimental model of bladder tumors induced by MNU in rats

作　　者：刘红耀[1] 李晓东[2] 米振国[1] 王东文[1] 李春华[3] 李柄太 佟锦[1] 

机构地区：[1]山西医科大学第一医院泌尿外科,太原030001 [2]太原理工大学化工系 [3]山西医科大学中心实验室 [4]山西医科大学中心电镜室

出　　处：《中华实验外科杂志》2000年第6期548-549,共2页Chinese Journal of Experimental Surgery

基　　金：山西省自然科学基金!资助项目 (9710 2 4)

摘　　要：目的　研究N 甲基亚硝基脲 (MNU)制备方法及其诱发大白鼠膀胱肿瘤的作用。方法由甲胺盐酸盐合成诱癌剂MNU ,进行大白鼠膀胱灌注 2mg/次 ,每 2周 1次 ,共 4次 ,以光镜、扫描电镜、透射电镜等为观察手段 ,对其诱发的膀胱肿瘤进行观察。结果　 8周诱发膀胱肿瘤率为 10 0 % ,其组织学检查及病理学特征与人膀胱肿瘤十分相似。结论　自制的MNU诱发的大白鼠膀胱肿瘤为比较理想的动物模型。Objective\ To study preparation of N methyl N nitrosourea (MNU) and development of the experimental animal model of bladder tumors induced by MNU in rats. Methods\ Bladder tumors were induced in femal wistar rats by intravascular administration of the carcinogen, MNU that was synthesized with CH 3NH 2HCL. The morphological alterations in bladder epithelial cells were studied under light microscopy, scanning and transmission electron microscopy. Results\ The administration way of a total dose of 8 mg on 4 fraction of 2 mg at 2 weekly intrvals produced a 100 % incidence of bladder tumor after 8 weeks. Histopathological characteristics of bladder tumor induced by MNU were basically similar to those of human bladder tumor. Conclusion\ Intravascular administration of MNU in rats provided a useful model for experimental studies on bladder tumor.\;

关 键 词：化学致癌剂 N-甲基亚硝基脲 动物模型 膀胱肿瘤 

分 类 号：R737.14[医药卫生—肿瘤]