阿霉素磁液术前靶向化疗对进展期胃癌细胞凋亡和增殖的影响  

The effect of adriamycin magnetic fluid targeting chemotherapy before operation on apoptosis and proliferaton of advanced gastric cancer cells

作　　者：陶凯雄[1] 陈道达[1] 吴在德[2] 田源[1] 王国斌[1] 杨秀萍[1] 

机构地区：[1]同济医科大学附属协和医院普外科,武汉430022 [2]同济医科大学附属同济医院普外科

出　　处：《中华实验外科杂志》2000年第6期561-562,共2页Chinese Journal of Experimental Surgery

基　　金：国家"九五"重点医学科技攻关项目!(96 90 6 0 116 );卫生部临床学科重点建设项目!(6 716 8)

摘　　要：目的　研究进展期胃癌术前给予阿霉素磁液靶向化疗后 ,胃癌组织中细胞凋亡与增殖状态的变化。方法　随机选择 30例进展期胃癌 ,术前 2周给予阿霉素磁液靶向化疗 ,应用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记法 (TUNEL法 )和增殖细胞核抗原 (PCNA)免疫组织化学染色法 ,分别检测化疗前后胃癌组织及癌周正常粘膜组织中细胞凋亡指数 (AI)与增殖细胞核抗原的指数 (PI)。结果　术前阿霉素磁液化疗对正常粘膜组织的细胞凋亡指数 ,增殖指数影响不大 ,凋亡指数化疗前后分别为 (7.5± 2 .9) %、(8.1± 3 .1) % ;增殖指数化疗前后分别为 (15 .4± 4.7) %、(14.5± 3.8) % ,差异无显著性 (P>0 0 .5 ) ,但可显著增加癌组织的细胞调亡指数 ,并降低增殖指数 ,从而提高凋亡 /增殖比率 ,差异有非常显著性 (P <0 .0 1)。结论　术前阿霉素磁液靶向化疗可明显诱导胃癌细胞凋亡的增加 ,并抑制其增殖活性。Objective\ To study the apoptosis and proliferaton of advanced gastric cancer cells following preoperative targetingly chemotherapy with adriamycin magnetic fluid. Methods\ Thirty cases of advanced gastric cancers, selected randomly, received targeting chemotherapy with adriamycin magnetic fluid 2 weeks before gastrectomy. The apoptosis index (AI) and the proliferating cell nuclear antigen (PCNA) index (PI) were detected respectively in the tissue of gastric cancer and the normal gastric mucosa before and after the chemotherapy by the TUNEL staining method and immunohistochemical method of PCNA.Results\ The preoperative chemotherapy with adriamycin magnetic fluid had no significant effect on the AI and PI in the normal mucosa tissue surrounding the mass(P>0.05). But in the gastric cancer tissue the AI could be obviously increased and the PI reduced with the rate of AI/PI being improved ( P <0.01) . Conclusion\ The preoperatively targeting chemotherapy with adriamycin magnetic fluid could markedly induce the increase of apoptosis and inhibit the activity of proliferation in gastric cancer.\;

关 键 词：胃癌 阿霉素 磁性微球 靶向化疗 PCNA 

分 类 号：R735.2[医药卫生—肿瘤]