光敏剂磁性纳米粒子螯合剂在兔VX2肝转移癌模型中的生物学分布  

Biodistribution of photosensitizer-magnetic nanoparticle chelate complex in rabbits with VX2 metastatic hepatic carcinoma

作　　者：龚健[1] 刘煜昊[2] 葛海燕[3] 

机构地区：[1]同济大学附属第十人民医院普外科,上海市200072 [2]同济大学附属第十人民医院重症监护室,上海市200072 [3]同济大学附属东方医院胃肠外科,上海市200120

出　　处：《世界华人消化杂志》2013年第6期464-470,共7页World Chinese Journal of Digestology

摘　　要：目的:观察光敏剂磁性纳米粒子螯合物(photosensitizer-magnetic nanoparticle chelate complex,PMNCC)进入兔VX2肝转移癌模型体内的生物学分布情况.方法:成功制备PMNCC,建立兔Vx2肝转移癌模型.在成瘤后第16、18、20天经耳缘静脉注射PMNCC,第22天处死实验兔,取肿瘤及肝、脾、心、肺、肾组织,利用普鲁氏染色、原子吸收光谱分析及透射电镜成像检测PMNCC在动物体内的生物学分布情况.PMNCC在肿瘤组织内的摄取量明显高于肝脏、脾脏、肾脏等其他脏器组织,原子吸收光谱分析检测肿瘤组织中铁相对浓度为9.09mg/L±2.31mg/L,明显高于其他脏器组织(肝脏4.43mg/L±1.10mg/L、脾脏5.08mg/L±1.35mg/L、肾脏3.95mg/L±1.70mg/L、心脏3.88mg/L±0.93mg/L、肺脏4.08mg/L±1.02mg/L),差异均有显著统计学意义(P<0.01).结论:PMNCC在兔VX2肝转移癌模型体内主要被肿瘤组织摄取,PMNCC具有促进磁性纳米粒子进入肿瘤细胞内的能力.AIM： To observe the biodistribution of photosensitizer-magnetic nanoparticle chelate complex （PMNCC） in rabbits with VX2 metastatic hepatic carcinoma. METHODS： After PNMCC was successfully prepared, a rabbit model of VX2 metastatic hepatic carcinoma was developed. PMNCC was admin- istered via the ear vein on the 16th, 18th and 20th day after induction of metastatic carcinoma, and tumor-bearing rabbits were sacrificed on the 22nd day. Tissue specimens were obtained and the biodistribution of PMNCC was observed by Prussian blue staining, atomic absorption spectroscopy （AAS） and transmission electron microscopy （TEM）. RESULTS： Prussian blue staining and TEM analysis showed that the amount of PMNCC in tumor tissue was significantly higher than those in the liver, spleen, or kidney. The mean relative iron concentration detected by atomic absorption spectroscopy in tumor tissue was 9.09 mg/L ± 2.31 mg/L, significantly higher than those in other organs （all P 〈 0.01）. CONCLUSION： PMNCC was mainly distributed in tumor tissue in rabbits with VX2 metastatic hepatic carcinoma, and PMNCC has the ability to carry magnetic nanoparticles into tumor cells.

关 键 词：光敏剂磁性纳米粒子螯合物 VX2转移癌 生物学分布 

分 类 号：R735.7[医药卫生—肿瘤]