CD99^+/mCD99L2^-淋巴瘤细胞亚系中CXCL16的表达及意义  

Expression of chemokine CXCL16 and its significance in CD99^+/mCD99L2^- subseries of lymphoma cell line

作　　者：张玉[1] 汤红平[1] 刘靖[2] 刘娜[2] 吴自勍[1] 刘芳[2] 赵彤[1] 

机构地区：[1]南方医科大学基础医学院病理系,广州510515 [2]佛山科学技术学院医学院生理病生教研室,佛山528000

出　　处：《临床与实验病理学杂志》2013年第2期122-125,130,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(青年科学基金81101537)

摘　　要：目的探讨趋化因子CXCL16在淋巴瘤细胞亚系(L428-CD99+和A20-mCD99L2-)中的表达,为深入研究CD99/mCD99L2基因对淋巴瘤细胞趋化因子的影响提供依据。方法分别提取各组细胞总RNA和蛋白,收集无血清培养细胞上清,应用QPCR、Western blot法和ELISA法分别检测各细胞组中CXCL16的表达并比较差异。结果 CXCL16 mRNA在L428-CD99+细胞中的表达低于对照组,在A20-mCD99L2-细胞中的表达高于对照组(P<0.05)。CXCL16总蛋白在L428-CD99+细胞中表达低于对照组,在A20-mCD99L2-细胞中表达高于对照组。ELISA法检测分泌型蛋白在L428-CD99+细胞中表达低于对照组,在A20-mCD99L2-细胞中表达高于对照组(P<0.05)。结论两组淋巴瘤细胞亚系中CXCL16表达与CD99/mCD99L2基因表达呈负相关,提示CD99/mCD99L2基因表达的变化可能影响淋巴瘤细胞该趋化因子的表达。Purpose To detect the expression of CXCL16 in previously established subseries of lymphoma cell lines （L428-CD99 ＋and A20-mCD99L2 ） in order to facilitate further researches into the effect of CD99/mCD99L2 gene on the chemokine secretion of lym- phoma cells. Methods Total RNA and protein were extracted and the mRNA and protein expression of CXCL16 were measured by quantitative PCR and Western blot. Supernatants of serum-free cultured cells were collected and the secreted CXCL16 protein was de- tected by ELISA. Results The CXCL16 mRNA in （L428-CD99 ＋ ） cell was significantly lower than that in the control group while it was much higher in （A20-mCD9912-） cell than that in the control （P 〈 0. 05）. The CXCL16 protein was lower in （IA28-CD99 ＋） cells and higher in （ A20-mCD99L2- ） cells than their controls, respectively. Secreted CXCL16 detected by ELISA was significantly lower in （ IA28-CD99 ＋ ） cell than that of the control while it was much higher in （A20-mCD99L2） cell than in the control group （P 〈 0. 05）. Conclusion The expression of CXCL16 and CD99/mCD99L2 in the two subseries of lymphoma cell lines is negatively cor- related, suggesting that regulating CD99/mCD99L2 possibly affects the chemokine （CXCL16） expression in the lymphoma cells.

关 键 词：霍奇金淋巴瘤 CD99基因 mCD99L2基因 趋化因子 CXCL16 

分 类 号：R730.23[医药卫生—肿瘤]