胰腺疾病组织N—myc下游调节基因2的表达与分布  

Expression and distribution of N-myc downstream regulated gene 2 in pancreatic diseases

作　　者：贺菲菲[1] 焦凯[1] 刘新平[2] 沈岚[2] 

机构地区：[1]第四军医大学唐都医院内分泌科,西安710038 [2]第四军医大学生物化学与分子生物学教研室

出　　处：《中华内分泌代谢杂志》2013年第2期162-164,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（31171112）

摘　　要：分离胰岛细胞增生症患者、胰岛素瘤患者、胰尾高中分化腺癌患者和正常人胰腺组织，制成石蜡切片，用抗N-myc下游调节基因2（Ndrg2）单克隆抗体进行免疫组织化学染色（ABC法），用Western印迹法检测Ndrg2的表达与分布情况。结果显示，Ndrg2阳性反应物主要分布在胰岛细胞的胞浆，与胰岛素的阳性反应物分布与定位相似；胰岛细胞增生症患者胰岛数量增加，体积增大，且该疾病患者胰腺中Ndrg2表达增加；Western印迹实验结果显示胰岛细胞增生症患者胰腺组织中Ndrg2蛋白的表达量较正常组增高。提示Ndrg2基因可能在胰岛细胞中发挥重要的生理功能。The pancreatic tissues from patients with islet cell hyperplasia, insulinoma, and pancreatic adenocarcinoma, as well as normal pancreatic tissues were embedded with paraffin, serial sections were cut and mounted on glass slides. Immunohistochemical staining was carried out with N-myc down-stream regulated gene 2 （Ndrg2） monoclonal antibody by means of ABC method, and Western blotting was carried out to detect the expression and distribution of Ndrg2. The results showed that Ndrg2 positive immunoreactivity was mainly localized in the cytoplasm of islet cell, being similar to the localization of insulin positive immunoreactivity. The number and volume of pancreatic islets were increased in the patients with islet cell hyperplasia, and Ndrg2 expression was also increased. Western blotting results showed that the expression of Ndrg2 in the pancreas of patients with islet cell hyperplasia was increased compared with normal group. The above results suggest that Ndrg2 may play an important role in performing physiological function of islet cells.

关 键 词：N—myc下游调节基因2 胰岛细胞 胰岛素 免疫组织化学 

分 类 号：R576[医药卫生—消化系统]