LBH589对人上皮性卵巢癌OVCAR-3细胞增殖的抑制作用及机制探讨  

Inhibitory function of LBH589 on the in vitro proliferation of human ovarian cancer cell lines and its mechanism

作　　者：晁宏图[1,2] 邓君丽[1,2] 马一鸣[1,2] 王莉[1,2] 

机构地区：[1]河南省肿瘤医院妇瘤科 [2]郑州大学附属肿瘤医院,郑州市450003

出　　处：《中国肿瘤临床》2013年第3期131-133,共3页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨新型组蛋白去乙酰化酶抑制剂LBH589对人上皮性卵巢癌OVCAR-3细胞增殖抑制和促进凋亡作用及其机制。方法:不同浓度LBH589处理细胞后,采用噻唑蓝(MTT)比色法检测对细胞增殖的影响;AO/EB(台盼蓝、吖啶橙溴化乙啶双染色法)检测细胞凋亡;Western blot检测聚腺苷二磷酸核糖聚合酶(PARP)、Caspase-3、Bcl-2、Bax蛋白水平。结果:LBH589明显抑制OVCAR-3细胞增殖,48 h半数抑制浓度(IC50)为0.12μM。Western blot检测发现Caspsae-3、PARP-85kD剪切蛋白增加,Bax表达增加,Bcl-2表达减少。结论:LBH589在体外条件下能明显抑制卵巢癌细胞OVCAR-3细胞增殖,诱导细胞凋亡。Objective：This study was designed to investigate the mechanism and effect of LBH 589, a novel histone deacetylase inhibitor, on the growth and apoptosis of the human ovarian cancer cell line OVCAR-3. Methods：The cells were treated with LBH589 at different concentrations. Cell proliferation was determined using the methyl thiazol tetrazolium assay. The apoptotic rate of the cells was detected by AO/EB double-staining. Protein expressions of polyADP-ribose polymerase （PARP）, caspase-3, bel-2, and bax were analyzed by western blot assay. Results：LBH 589 significantly inhibited the proliferation of OVCAR- 3 cells at a concentration of 0.12 μM/L based on a 48 h half-inhibitory concentration （IC 50 ）. Western blot assay showed that the expressions of cleaved PARP- 85 KD, caspase-3, and bax were increased, but bcl-2 expression was downregulated. Conclusion： LBH 589 in vitro can significantly inhibit the proliferation and induce apoptosis of the human ovarian cancer cell line OVCAR- 3.

关 键 词：LBH589 卵巢癌 细胞增殖 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]