新型真菌源激活蛋白诱导水稻抗病性及其生理机制  

Rice Disease Resistance Induced by New Fungal Activator Protein and Its Physiological Mechanism

作　　者：袁肖寒[1,2] 顾成波[1] 邱德文[3] 付丽楠[1] 李旺[1] 王秋雪[1] 郭东杰[1] 蔡曼[1] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]东北农业大学生命科学与生物技术研究中心,哈尔滨150030 [3]中国农业科学院植物保护研究所,北京100081

出　　处：《植物研究》2013年第2期220-224,共5页Bulletin of Botanical Research

基　　金：中央高校基本科研业务费专项资金资助(DL13CA02);黑龙江省高校农业生物功能基因重点实验室开放课题(NSGJ2012-02);哈尔滨市科技创新人才专项资金(2011RFQXS074)

摘　　要：为明确新型真菌源激活蛋白对水稻抗病性的诱导作用及其生理机制,研究了激活蛋白对水稻稻瘟病和白叶枯病的诱导抗病性,监测了激活蛋白处理后水稻过氧化物酶(POD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性及过氧化氢(H2O2)含量变化。结果表明,1～6μg.mL-1激活蛋白对稻瘟病和白叶枯病的诱抗效果分别为45.2%～71.4%和47.6%～66.3%,以6μg.mL-1激活蛋白的诱抗效果最好。与对照相比,2μg.mL-1激活蛋白处理水稻后3～15 d内不同程度诱导了防御酶POD、PPO和SOD活性,抑制CAT活性,提高H2O2含量。新型真菌源激活蛋白能够诱导水稻产生对稻瘟病和白叶枯病的抗病性,其诱导抗性机制与水稻体内的活性氧代谢密切相关。In order to clarify new fungal activator protein induced resistance in rice and its physiological mecha- nism, changes of peroxidase （POD）, polyphenol oxidase （POD）, superoxide dismutase （SOD） and catalase （CAT） activities and hydrogen peroxide （ H2O2 ） content in rice seedlings were determined at various time points after activator protein treatment. The results showed that activator protein could induce resistance in rice against blast fungus and bacterial blight. The resistance induced by activate protein at the concentration of 1 -6μg .mL-1 against blast fungus and bacterial blight was 45.2% -71.4% and 47.6% -66.3%, respectively, and the best results were achieved at the concentration of 6μg. mL-1 activate protein. Compared with control group, the ac- tivities of POD, PPO and SOD and hydrogen peroxide content in rice seedlings were increased with different levels, but CAT activities inhibited 3 -15 d after the rice seedlings treated with the concentration of 2μg.mL-1 activator protein. These findings showed that activator protein-induced resistance in rice was closely related to active oxygen metabolism.

关 键 词：激活蛋白 水稻病害 诱导抗性 活性氧代谢 

分 类 号：S511[农业科学—作物学]