马铃薯杂种F_1无性株系的ISSR鉴定  

ISSR Identification of Clone Plants of Solanum tuberosum Hybrids F_1

作　　者：甘霖[1] 于肖夏[2] 鞠天华 于卓[1] 蒙美莲[1] 张自强[1] 李长青[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]中国农业大学农业部作物遗传改良重点实验室,北京100193 [3]赤峰天泽生物科技有限公司,赤峰025457

出　　处：《植物研究》2013年第2期243-247,共5页Bulletin of Botanical Research

基　　金："十二五"国家科技支撑计划项目(2012BAD02B05);内蒙古粮食丰产科技工程项目(2012NMLF0016)资助

摘　　要：为给马铃薯新品种选育提供可靠材料,采用ISSR分子标记对2个马铃薯杂交组合‘J07-4’ב陇薯6号’和‘J07-6’ב陇薯6号’杂交种F1无性繁殖株系的真实性进行了鉴定。结果从152个ISSR引物中筛选出适于‘J07-4’ב陇薯6号’杂种F15个无性株系鉴定的3个ISSR引物AF18550、AW75511和AW20617及‘J07-6’ב陇薯6号’杂种F17个无性株系鉴定的2个ISSR引物AW20607和AF18549;以子代中含有父本特征带为主要依据,鉴定出杂种F1共12个无性繁殖株系均为真实的杂交种,并建立了杂种F1不同无性株系间的ISSR指纹图。表明ISSR分子标记技术用于马铃薯杂种F1无性株系鉴定是可行的。In order to provide reliable materials for potato varieties breeding, the authenticity of clones of hybrids F1 of two potato combinations, ‘J07-4＇ × ‘ Longsh study was to u No, 6＇ and identify the ‘ J07-6 ＇ב Longshu No. 6＇ , using ISSR molecular markers. The results were that five primers were selected from 152 ISSR primers, primers AF18550, AW75511 and AW20617 were suitable to identify 5 clones of ‘J07-4＇ ב Longshu No. 6＇ hybrids F1, and AW20607 and AF18549 were suitable to identify 7 clones of ‘ J07-6＇ × ‘ Longshu No. 6＇ hybrids F1. A total of 12 clones were identified as true hybrids based on the paternal-specific bands in progeny＇ s bands. ISSR fingerprints of different clones of hybrids F1 were established. It showed that it was feasible to use ISSR molecular markers to identify clones of potato hybrids F1.

关 键 词：马铃薯 杂种F1 无性株系 ISSR鉴定 

分 类 号：S532[农业科学—作物学] S336