过表达NNMT对SMMC7721肝癌细胞生物学行为的影响  

The Effect of Nicotinamide N-methytransferase Overexpression on Biological Behaviors of SMMC7721 Hepatocellar Carcinoma Cell Line

作　　者：牟霞[1,2] 陈瑶[1] 王穗海[1] 李明[1] 

机构地区：[1]贵州省人民医院医院感染管理科,贵阳550002 [2]南方医科大学生物技术学院抗体工程研究所,广州510515

出　　处：《四川大学学报（医学版）》2013年第2期193-195,217,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家高技术研究发展计划863项目(No.2006AA02A311)资助

摘　　要：目的探讨尼克酰胺-N-甲基化酶(NNMT)对人肝癌细胞株SMMC7721恶性生物学行为影响。方法分别对转染NNMT基因SMMC7721/NNMT组(简称S/NNMT)、对照组SMMC7721细胞组(简称S)及转染pcDNA3.1空质粒SMMC7721/pcDNA3.1(简称S/P)组细胞进行MTT增殖实验、克隆形成实验、黏附实验、侵袭实验等,以研究过表达NNMT对细胞生物学行为的影响。结果 MTT法检测结果显示S/NNMT组细胞增殖速度、克隆形成率与S组、S/P组比较差异无统计学意义(P>0.05),S/NNMT组细胞异质黏附能力明显高于S组、S/P组细胞(P<0.001);Transwell侵袭力实验结果显示S/NNMT组细胞OD值明显高于其余两组(P<0.001)。结论 NNMT可以使肝癌细胞的恶性表形发生变化,使其侵袭转移能力增强。Objective To study the effect of over expressed nicotinamide N-methytransferase （NNMT） on the malignant bioJogical behaviors of hepatocelluiar carcinoma cell line SMMC7721. Methods The cells transfected with SMMC/NNMT abbreviated as S/NNMT, parental cells including the negative control which transfected with SMMC/pcDNA3. 1 abbreviated as S/P and parental group which abbreviated S. MTT assay and Plate Clone Formation Assay, cell adhension assay and invasion assay were applied to evaluate the effect of NNMT overexpression on the malignant biological behaviors of hepatocellular carcinoma cell line SMMC7721. Results Compared with the negative control and parental group, MTT assay and Plate Clone Formation Assay demonstrated that over expression of NNMT in SMMC7721 cells could not influence the proliferation clone formation of SMMC7721 cells （P）O. 05）. Over expression of NNMT in SMMC7721 cells significantly enhanced the ability of invasion and adhension （P〈0. 001）. Conclusion NNMT gene can partially influence the malignant behaviors of hepatocellular carcinoma cellline SMMC7721 and increased the ability of invasion and adhension.

关 键 词：尼克酰胺-N甲基化酶 肝癌细胞 侵袭力 黏附能力 

分 类 号：R735.7[医药卫生—肿瘤]